Molecular Genetic Identification of <em>Fomitopsis</em> spp. Fungi
from the Irkutsk Region

E. A. Orlova; D. V. Zakharova; B. N. Ogarkov; S. N. Zhdanova,O. B. Ogarkov

List of issues > Series «Biology. Ecology». 2022. Vol 40

Molecular Genetic Identification of Fomitopsis spp. Fungi from the Irkutsk Region

Author(s)

E. A. Orlova, D. V. Zakharova, B. N. Ogarkov, S. N. Zhdanova,O. B. Ogarkov

Abstract

Basidiomycetes of the genus Fomitopsis are an extensive group of more than 100 species that have ecosystem significance and high biotechnological potential. One of these species, Fomitopsis pinicola, is known as a widespread tinder fungus, a saprophyte that causes brown rot on deciduous and coniferous trees. The modern taxonomy of F. pinicola, economic significance, relevance of pharmacological research and the possibility of using them as medicines and food additives are discussed. F. pinicola has an important ecosystem function as it promotes the nutrient circulation in the forest. Recent studies show that this is a species complex and that morphological markers alone are not sufficient for precise identification of the species, which emphasises the importance of a molecular genetic approach to the taxonomy of this group. Although F. pinicola can be detrimental to forestry by causing rotting of wood, this basidiomycete has long been used by humans. For a long time, extracts of the conks (fruiting bodies) of F. pinicola, like many other tinder fungi, have been used by various cultures in traditional medicine, but modern pharmacological studies of the health-saving properties of the substances produced by F. pinicola are still insufficient. However, the relevance of such studies is very high, since ergosterol, fomitopic acids, and fomitosides produced by fungi demonstrate a proapoptotic effect and affect the metabolic pathway of prostaglandins. Promisingly, some compounds isolated from other Fomitopsis species have anticancer properties. Despite this, in fungal taxonomy, the correct species identification of biotechnological strains remains a problem. Due to intraspecific morphological variation, modern taxonomy emphasizes genetic markers. Here the data of molecular-genetic and phylogenetic identification of two biotechnological strains of basidiomycetes collected from the affected larch stands of the Irkutsk region are presented. Genetic identification of strains of Fomitopsis spp. was carried out using the sequence of variable regions of the ITS1 ribosomal operon. For phylogenetic analysis, ITS1 gene sequences of 25 species of basidiomycetes evolutionarily closest to Fomitopsis spp. were used. The studied strains were identified as the F. pinicola species with an accuracy of 97.98% and 99.21%, respectively. Further study of the biotechnological properties of the two identified strains of F. pinicola seems to be very relevant.

About the Authors

Orlova Elizaveta Andreevna, Junior Research Scientist, Scientific Centre of Family Health and Human Reproduction Problems, 16, Timiryazev st., Irkutsk, 664003, Russian Federation, e-mail: elizaveta.a.orlova@gmail.com

Zakharova Daria Vyacheslavovna, Undergraduate, Irkutsk State University, 1, K. Marx st., Irkutsk, 664003, Russian Federation, e-mail: darya.zaxarova.98@inbox.ru

Ogarkov Boris Nikitovich, Doctor of Sciences (Biology), Professor, Head of Laboratory, Irkutsk State University, 1, K. Marx st., Irkutsk, 664003, Russian Federation, e-mail: bornik@bk.ru

Zhdanova Svetlana Nikolaevna, Doctor of Sciences (Medicine), Leading Research Scientist, Scientific Centre of Family Health and Human Reproduction Problems, 16, Timiryazev st., Irkutsk, 664003, Russian Federation, e-mail: svetnii73@gmail.com

Ogarkov Oleg Borisovich, Doctor of Sciences (Medicine), Head of Department, Scientific Centre of Family Health and Human Reproduction Problems, 16, Timiryazev st., Irkutsk, 664003, Russian Federation, e-mail: obogarkov@yandex.ru

For citation

Orlova E.A., Zakharova D.V., Ogarkov B.N., Zhdanova S.N., Ogarkov O.B. Molecular Genetic Identification of Fomitopsis spp. Fungi from the Irkutsk Region. The Bulletin of Irkutsk State University. Series Biology. Ecology, 2022, vol. 40, pp. 15-23. https://doi.org/10.26516/2073-3372.2022.40.15 (in Russian)

Keywords

basidiomycetes, Fomitopsis pinicola, ITS1 16SrRNA, Sanger sequencing, phylogenetic analysis.

UDC

57.065

DOI

https://doi.org/10.26516/2073-3372.2022.40.15

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List of issues > Series «Biology. Ecology». 2022. Vol 40

Molecular Genetic Identification of Fomitopsis spp. Fungi from the Irkutsk Region

Full text (russian)