The capacity of biofuel elements based on Clostridium acetobutylicum strain VKPM-B-4786 was determined and compared. A model of a two- chamber biofuel element made of plexiglas consisting of a hermetically sealed anodic and aerobic cathode chamber was used in the work. Access to the anolyte biofuel element during its continuous operation, without compromising sterility and anaerobic content (for sampling and depositing substrates and bioagents) was carried out through a special rubber plug in the side of the anode chamber. Carbon fabric URAL T-22P A (OJSC «Svetlogorsk Khimvolokno», Belarus) was used as electrodes. The anolyte in biofuel elements was a potato medium, a liquid medium for clostridium [8], model wastewater with the addition of various substrates and wastes (starch, peptone, glucose, cellulose, birch sawdust). The obtained experimental data made it possible to align the test media with the corresponding substrates in the next row (in terms of the power generated by Cl. acetobutylicum): a liquid medium for clostridium (10.4±1.2 μW /cm²) > potato medium (8.7±0.9 μW/cm²) > birch sawdust (6.2±0.7 μW/cm²) > cellulose (2.3±0.3 μW/cm²) > peptone (2.1±0.2 μW/cm²) > starch (1.1±0.1 mW/cm²) > glucose (0.3±0.09 mW/cm²). Generation of electricity in biofuel cells was accompanied by an increase in the number of cells Cl. acetobutylicum. The results of the experiments demonstrate the fundamental possibility of generating electricity in biofuel cells by the production strain Cl. acetobutylicum VKPM-B-4786 and allow us to recommend the use of this strain for producing electricity in biofuel technology when recycling potato waste and processing wood. 

Gorbunova Yuliya Olegovna, Research Assistant, Irkutsk State University, 1, K. Marx st., Irkutsk, 664003, Russian Federation, tel.: (3952) 34–34–37, e-mail: iuliia_gorbunova_93@mail.ru 

Zhdanova Galina Olegovna, Research Scientist, Irkutsk State University, 1, K. Marx st., Irkutsk, 664003, Russian Federation, tel.: (3952) 34–34–37, e-mail: zhdanova86@yandex.ru 

Tsyrenov Vladimir Zhigzhitovich, Doctor of Sciences (Biology), Professor, East-Siberian State University of Technology and Management, 40B, building 1, Klyuchevskaya st., Ulan-Ude, Republic of Buryatia, 670013, Russian Federation, tel.: (3012)43–14–15, (3012)41–71–50, e-mail: vtsyrenov@gmail.com 

Stom Devard Iosifovich, Doctor of Sciences (Biology), Professor, Head of laboratory, Irkutsk State University, 1, K. Marx st., Irkutsk, 664003, Russian Federation, Professor, Irkutsk National Research Technical University, 83, Lermontov St., Irkutsk, 664074, Russian Federation, Chief Research, Scientist Baikal Museum ISC SB RAS, 1, Akademicheskaya st., Listvyanka Settl., 664520, Russian Federation, tel.: (3952) 34–34–37, e-mail: stomd@mail.ru 

Chroni Mikhail Eduardovich, Student, Irkutsk National Research Technical University, 83, Lermontov St., Irkutsk, 664074, Russian Federation, tel.: (3952) 40–51–06, e-mail: ya.misha20122@yandex.ru 

Tolstoy Mikhail Yurievich, Candidate of Sciences (Technics), Head of Chair, Director of Energy Efficiency Innovation Center by INRTU, Irkutsk National Research Technical University, 83, Lermontov St., Irkutsk, 664074, Russian Federation, tel.: (3952) 40-51-43, (3952) 40-51-42, e-mail: tolstoi@istu.edu 

Ryabchikova Irina Alekseevna, Candidate of Sciences (Biology), Associate Professor, Irkutsk National Research Technical University, 83, Lermontov St., Irkutsk, 664074, Russian Federation, tel.: (3952) 40–51–06, e-mail: rjabchik@bk.ru 

Fialkov Vladimir Abramovich, Candidate of Sciences (Geography), Leading Research Scientist, Adviser to the Chairman of SB RAS, Baikal Museum ISC SB RAS, 1, Academicheskaya st., Listvyanka Settl., 664520, Russian Federation, tel .: (3952) 45–31–46, e-mail: fva@isc.irc.ru 

Kupchinsky Alexander Borisovich, Candidate of Sciences (Biology), Acting Director, Baikal Museum ISC SB RAS, 1, Academicheskaya st., Listvyanka Settl., 664520, Russian Federation, tel .: (3952) 45–31–46, e-mail: albor67@mail.ru 

Sanket Goel, PhD, Head of Department Birla, Institute of Technology and Science, Pilani Hyderabad Campus, Jawahar Nagar, Shameerpet, Hyderabad, 500078, India, tel.: 040–66303686, e-mail: sgoel@hyderabad.bits-pilani.ac.in

Gorbunova Yu.O., Tsyrenov V.Zh., Zhdanova G.O., Stom D.I., Chroni M.E., Tolstoy M.Yu., Ryabchikova I.A., Fialkov V.A., Kupchinskiy A.B., Goel S. Clostridium acetobutylicum as a Bioagent in Biofuel Cells. The Bulletin of Irkutsk State University. Series Biology. Ecology, 2018, vol. 24, pp. 16-24. https://doi.org/10.26516/2073-3372.2018.24.16 (in Russian)

biofuel cells (BFC), Clostridium acetobutylicum, electricity, bioagent

Lashin A.F., Borokhoev N.D., Krasavtseva M.S., Stom D.I., Zhdanova G.O., Tolstoi M. Yu. Bioelektrokhimicheskii element [Bioelectrochemical element] Patent (poleznaya model') RF № 170868 ot 05.07.2016. [RF patent for utility model No. 170868 dd. 05.07.2016.] (in Russian)

Berezina O.V., Sineokii S.P., Velikodvorskaya G.A., Shvarts B., Zverlov V.V. Vnekletochnaya glikozilgidrolaznaya aktivnost' klostridii, obrazuyushchikh atseton, butanol i etanol [Extracellular glycosyl hydrolase activity of clostridia forming acetone, butanol and ethanol], Prikladnaya biokhimiya i mikrobiologiya [Applied Biochemistry and Microbiology], 2008, vol. 44, pp. 49–55. (in Russian)

Stom D.I., Konovalova E.Yu., Zhdanova G.O., Tolstoy M.Yu., Vyatchina O.F. Active sludge and strains isolated from it as bioagents in biofuel cells. SGEM 2017 Conf. Proc, 2017, vol. 17, no. 42, pp. 19–26. https://doi.org/10.5593/sgem2017/42/S17.003

Cheng H.-H., Whang L.-M., Chan K.-C., Chung M.-C., Wu S.-H., Liu C.-P., Tien S.-Y., Chen S.-Y., Chang J.-S., Lee W.-J. Biological butanol production from microalgae-based biodiesel residues by Clostridium acetobutylicum. Bioresource Technology, 2015, vol. 184, pp. 379–385.

Liu X.W., Li W.W., Yu H.Q. Cathodic catalysts in bioelectrochemical systems for energy recovery from wastewater. Chem. Soc. Rev, 2014, vol. 43, pp. 7718–7745.

Grigorova R., Norris J. R. (eds.). Methods in Microbiology. 1990, vol. 22, 618 p.

Logan B.E., Aelterman P., Hamelers B., Rozendal R., Schröer U., Keller J., Freguia S., Verstraete W., Rabaey K. Microbial fuel cells: methodology and technology. Environ. Sci. Technol, 2006, vol. 40, pp. 5181–5192.

Ibrahim M.F., Abd-Aziz S., Razak M.N., Phang L.Y., Hassan M.A. Oil palm empty fruit bunch as alternative substrate for acetone-butanol-ethanol production by Clostridium butyricum EB6. Appl. Biochem. Biotechnol, 2012, vol. 166, pp. 1615–1625.

Gogoi H., Nirosha V., Jayakumar A., Prabhu K., Maitra M., Panjanathan R. Paper mill sludge as a renewable substrate for the production of acetone-butanol-ethanol using Clostridium sporogenes NCIM 2337. Energy Sources Part A: Recovery, Utilization, and Environmental Effects, 2018, vol. 40, no. 1, pp. 39–44. https://doi.org/10.1080/15567036.2017.1405107

Spiegelman D., Whissell G., Greer C.W. A survey of the methods for the characterization of microbial consortia and communities. Can. J. Microbiol, 2005, vol. 51, pp. 355–386.