рус eng
«IZVESTIYA IRKUTSKOGO GOSUDARSTVENNOGO UNIVERSITETA». SERIYA «BIOLOGIYA. ECOLOGIYA»
«THE BULLETIN OF IRKUTSK STATE UNIVERSITY». SERIES «BIOLOGY. ECOLOGY»
ISSN 2073-3372 (Print)

List of issues > Series «Biology. Ecology». 2023. Vol 46

Variability of Morphological and Genetic Characteristics of Macrohectopus branickii (Dyb., 1874) (Amphipoda, Macrohectopidae)

Author(s)
J. V. Petunina, N. V. Vavrischuk, Yu. S. Bukin, E. V. Romanova
Abstract
Macrohectopus branickii is the only pelagic amphipod from Lake Baikal. Until now, despite the uniqueness of M. branickii, its genetic diversity at the intraspecific level, as well as its intraspecific morphological diversity, have not been studied. In this work, we present the result of a morphological analysis of intrapopulation variability of the species M. branickii from the northern basin of Lake Baikal, as well as the result of a molecular analysis based on the sequence of a fragment of the mitochondrial Nad5 gene of several individuals of this species from three basins of the lake. The material for the study was collected using a plankton net and trawl from all basins and shores of Lake Baikal at depths of 100-300 m. We measured 21 morphological characters according to the scheme previously proposed in Tikhonova’s work for sexually mature females of M. branickii living on different shores of the northern basin of Lake Baikal. The coefficient of variation of the values of all characteristics was determined. Morphological analysis revealed different degrees of variability in the studied characters for M. branickii. The most variable characters are: the length of 1 segment of the peduncle of the upper antennae; length of 4 and 5 segments of the stalk of the lower antennae; length of gnathopod propodites 2 pairs; width and length of basipodites of the third pair of pereiopods; length of the stalk, external and internal branches of the uropods of the third pair; telson width and length. Analysis of the nucleotide sequences of the Nad5 gene fragment showed their low variability. The haplotype network was reconstructed using the pegas package for the R programming language. M. branickii forms a single panmictic population, consistent with the absence of geographic and environmental barriers in the pelagic zone of the lake.
About the Authors

Petunina Zhanna Vladimirovna, Candidate of Science (Biology), Junior Research Scientist, Limnological Institute SB RAS, 3, Ulaanbaatar st., Irkutsk, 664033, Russian Federation, e-mail: petuninajmk@mail.ru

Vavrischuk Nikolay Vladimirovich, Undergraduate, Irkutsk State University, 1, K. Marx st., Irkutsk, 664003, Russian Federation, e-mail: vavrischuck.nickolaa@yandex.ru

Bukin Yuri Sergeevich, Candidate of Science (Biology), Senior Research Scientist, Limnological Institute SB RAS, 3, Ulaanbaatar st., Irkutsk, 664033, Russian Federation, Associate Professor, Irkutsk State University, 1, K. Marx st., Irkutsk, 664003, Russian Federation, e-mail: bukinyura@mail.ru

Romanova Elena Vladimirovna, Candidate of Science (Biology), Research Scientist, Limnological Institute SB RAS, 3, Ulaanbaatar st., Irkutsk, 664033, Russian Federation, e-mail: era_85@inbox.ru

For citation
Petunina1 J. V., Vavrischuk N. V., Bukin Yu. S., Romanova E. V. Variability of Morphological and Genetic Characteristics of Macrohectopus branickii (Dyb., 1874) (Amphipoda, Macrohectopidae). The Bulletin of Irkutsk State University. Series Biology. Ecology, 2023, vol. 46, pp. 18-28. https://doi.org/10.26516/2073-3372.2023.46.18 (in Russian)
Keywords
morphometry, population genetic analysis, amphipods, Macrohectopus branickii, Lake Baikal.
UDC
595.2:595.36
DOI
https://doi.org/10.26516/2073-3372.2023.46.18
References

Bazikalova A.Ya. Amfipody ozera Baikal [Amphipods of Lake Baikal]. Trudy Baikal'skoi limnologicheskoi stantsii [Proc. Baikal Limnol. St.], 1945, vol. 11, pp. 1-440. (in Russian)

Grant V. Evolyutsionnyi protsess [Evolutionary Process]. Moscow, Mir Publ., 1991, 488 p. (in Russian)

Kuz'menkova Zh.V., Shcherbakov D.Yu., Smit D.E. Raznoobrazie mikrosporidii, parazitiruyushchikh na baikal'skikh amfipodakh Gmelinoides fasciatus iz raznykh populyatsii [Diversity of microsporidia parasitizing the Baikal amphipod Gmelinoides fasciatus from different populations]. Bull. Irkutsk St. Univ. Ser Biol. Ecol., 2008, vol. 1, no. 2, pp. 56-61. (in Russian)

Takhteev V.V. Ocherki o bokoplavakh ozera Baikal [Essays on the amphipods of Lake Baikal]. Irkutsk, Irkutsk St. Univ. Publ., 2000, 355 p. (in Russian)

Tikhonova E.N. Izmenchivost populyatsii Pallasea cancellus (Pallas, 1772) (Crustacea, Amphipoda) v ozere Baikal i reke Angara [Variability of Pallasea cancellus (Pallas, 1772) (Crustacea, Amphipoda) populations in Lake Baikal and the Angara River]. Cand. sci. diss. abstr. Novosibirsk, 2012, 152 p. (in Russian)

Bekman M.Y., Afanasyeva E.L. Distribution and production of Macrohectopus. Proc. Limnol. Inst. Vol. 19. Biological Productivity of Baikal Pelagic Region and Its Dynamics. Novosibirsk, Nauka Publ., 1977, pp. 76-98.

Doyle J.J., Dickson E.E. Preservation of plant samples for DNA restriction endonuclease analysis. Taxon, 1987, vol. 36, is. 4, pp. 715-722. https://doi.org/10.2307/1221122

Romanova E.V., Aleoshin V.V., Kamaltynov R.M., Mikhailov K.V., Logacheva M.D., Sirotinina E.A., Gornov A.Yu., Anikin A.S., Sherbakov D.Y. Evolution of mitochondrial genomes in Baikalian amphipods. BMC genomics, 2016, vol. 17, no. 14, pp. 291-306.https://doi.org/10.1186/s12864-016-3357-z

Hall T. BioEdit: An important software for molecular biology. GERF Bull. Biosci., 2011. vol. 2, is. 1, pp. 60-61.

Enard W., Khaitovich Ph., Klose J., Zöllner S., Heissig F., Giavalisco P., Nieselt-Struwe K., Muchmore E., Varki A., Ravid R., Doxiadis G.M., Bontrop R.E., Pääbo S. Intra-and interspecific variation in primate gene expression patterns. Science, 2002, vol. 296, is. 5566, pp. 340-343.https://doi.org/10.1126/science.1068996

Ishikawa T., Urabe J. Population dynamics and production of Jesogammarus annandalei, an endemic amphipod, in Lake Biwa, Japan. Freshw. Biol., 2002, vol. 47, is. 10, pp. 1935-1943.https://doi.org/10.1046/j.1365-2427.2002.00942.x

Macdonald III K.S., Yampolsky L., Duffy J.E. Molecular and morphological evolution of the amphipod radiation of Lake Baikal. Mol. Phylog. Evol., 2005, vol. 35, no. 2, pp. 323-343.https://doi.org/10.1016/j.ympev.2005.01.013

Karnaukhov D.Y., Dolinskaya E.M., Biritskaya S.A., Teplykh M.A., Ermolaeva Ya.K., Pushnica V.A., Kuznetsova I.V., Okholina A.I., Bukhaeva L.B., Rusanovskaya O.O., Silow E.A. New data regarding ecology of freshwater pelagic amphipod Macrohectopus branickii (Dybowsky, 1874) (Amphipoda: Macrohectopodidae) and other crustaceans of plankton from the southern part of Lake Baikal (Russia, Southern Siberia). Acta Biologica Sibirica, 2021, vol. 7, pp. 39-48.https://doi.org/10.3897/abs.7.e65636

Paradis E. Pegas: an R package for population genetics with an integrated–modular approach. Bioinformatics, 2010, vol. 26, no. 3, pp. 419-420. https://doi.org/10.1093/bioinformatics/btp696

Sanvicente-Añorve L., Ramírez-Velázquez B., Hermoso-Salazar M. Pelagic amphipods (Crustacea, Amphipoda, Hyperiidea) from the southern Gulf of Mexico with notes on the distribution of species. Biodiv. Data J., 2023, vol. 11, e97347. https://doi.org/10.3897/BDJ.11.e97347

Romanova E.V., Aleoshin V.V., Kamaltynov R.M., Mikhailov K.V., Logacheva M.D., Sirotinina E.A., Sherbakov D.Y. The mitochondrial genome of a freshwater pelagic amphipod Macrohectopus branickii is among the longest in Metazoa. Genes, 2021, vol. 12, 2030.https://doi.org/10.3390/genes12122030

Terry R.S., Smith J.E., Sharpe R.G., Rigaud T., Littlewood D.T.J., Ironside D., Rollinson D., Bouchon C., MacNeil J.T.A, Dick J.E., Dunn A.M. Widespread vertical transmission and associated host sex-ratio distortion within the eukaryotic phylum Microspora. Proc. Roy. Soc. London, Ser. B, Biol. Sci., 2004, vol. 271 (1550), pp. 1783-1789. https://doi.org/10.1098/rspb.2004.2793

Witt J.D., Blinn D.W., Heber P.D. The recent evolutionary origin of the phenotypically novel amphipod Hyalella montezuma offers an ecological explanation for morphological stasis in a closely allied species complex. Mol. Ecol., 2003, vol.12, is. 2, pp. 405-413. https://doi.org/10.1046/j.1365-294x.2003.01728.x

Wittner M., Weiss L. M. The Microsporidia and Microsporidiosis. ASM-Press, 1999, 553 p.


Full text (russian)