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«IZVESTIYA IRKUTSKOGO GOSUDARSTVENNOGO UNIVERSITETA». SERIYA «BIOLOGIYA. ECOLOGIYA»
«THE BULLETIN OF IRKUTSK STATE UNIVERSITY». SERIES «BIOLOGY. ECOLOGY»
ISSN 2073-3372 (Print)

List of issues > Series «Biology. Ecology». 2023. Vol 43

Evaluation of the Effects of Growth Stimulation of the Bifidobacterium with Polysaccharides from Siberian Larch

Author(s)
A. E. Makarova, G. V. Yurinova, Yu. P. Dzhioev, B. G. Sukhov, A. A. Pristavka, G. A. Teterina, N. A. Arefieva, A. Yu. Borisenko, V. P. Salovarova
Abstract
Due to the rich chemical composition of Siberian larch (Larix sibirica Ledeb), its products are widely used in medicine, pharmacology, agriculture, perfumery and cosmetics industry. Larch wood and bark contain a large amount of low molecular weight substances with high biological activity, namely: essential oils, ascorbic acid, tannins, anthocyanins, flavonoids, organic acids. The most important and useful of them are flavonoids due to their significant content in raw materials such compounds as: dihydroquercetin (DHA), arabinogalactan (AG), quercetin (QU), ardixin (ARD). On the basis of dihydroquercetin, preparations for the treatment and prevention of atherosclerosis, capillary-strengthening agents, anti-allergic drugs are created, and due to the high ability to complex formation, it actively removes heavy metals, including radionuclides, from the body. The main experimentally established properties of AG are immunomodulatory and antioxidant effects. In the diet, there is often a deficiency of dietary fiber needed by the human body. Quercetin is a powerful antioxidant that increases the body's resistance to radiation and a food antioxidant. It can also enhance antiinflammatory, antiviral, and immunoprotective effects. Ardiksin is a nanobiocomposite containing no. more than 95.0% of the natural polysaccharide arabinogalactan and no. less than 5% of the bioflavonoid dihydroquercetin. The biological activity of ardixin has shown its effectiveness in the treatment of oncological diseases as a concomitant agent during radiation and chemotherapy. Based on the significant importance of these polysaccharide compounds in the creation of new functional food products and therapeutic agents for medicine, in this study, the goal was to identify the effect of stimulating the growth of the commercial probiotic preparation Bifidobacterium bifidum when cultivated on media with polysaccharide compounds: purified arabinogalactan, dihydroquercetin, quercetin, ardixin. These polysaccharide compounds are processed products of Siberian larch (Larix sibirica Ledeb). In the course of a study on the evaluation of growth stimulation of B. bifidum by polysaccharides: AG-purified, DHA, QU, ADC, it was found that: 1) growth of B. bifidum is most effective in a medium with DHA; 2) when growing on a medium with AG-clean. there is almost five times less growth stimulation compared to DHA; 3) the smallest growth of B. bifidum is observed in the medium with QU, which is more than 208 times less than its growth on the medium with DHA.
About the Authors

Makarova Angelina Eduardovna, Student, Irkutsk State Medical University, 1, Krasnogo Vosstaniya st., Irkutsk, 664003, Russian Federation, e-mail: eamak18@mail.ru

Yurinova Galina Valerievna, Candidate of Sciences (Biology), Associate Professor, Irkutsk State University, 1, K. Marx st., Irkutsk, 664003, Russian Federation, e-mail: yurinova@yandex.ru

Dzhioev Yuri Pavlovich, Candidate of Sciences (Biology), Senior Research Scientist, Irkutsk State Medical University, 1, Krasnogo Vosstaniya st., Irkutsk, 664003, Russian Federation, e-mail: alanir07@mail.ru)

Sukhov Boris Gennadievich, Candidate of Sciences (Chemistry), Leading Research Scientist, V. V. Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, 3, Institututskaya st., Novosibirsk, 630090, Russian Federation, e-mail: boris_sukhov@mail.ru

Pristavka Aleksey Aleksandrovich, Candidate of Sciences (Biology), Associate Professor, Irkutsk State University, 1, K. Marx st., Irkutsk, 664003, Russian Federation, e-mail: pristavk@gmail.com

Teterina Galina Alexandrovna, MMR Specialist, Irkutsk State University, 1, K. Marx st., Irkutsk, 664003, Russian Federation, e-mail: galina.teterina.91@mail.ru

Arefieva Nadezhda Alexandrovna, Postgraduate, Irkutsk State Medical University, 1, Krasnogo Vosstania st., Irkutsk, 664003, Russian Federation, e-mail: arefieva.n4@gmail.com

Borisenko Andrey Yurievich, Candidate of Sciences (Biology), Senior Lecturer, Irkutsk State Medical University, 1, Krasnogo Vosstania st., Irkutsk, 664003, Russian Federation, e-mail: 89500720225@mail.ru

Salovarova Valentina Petrovna, Doctor of Sciences (Biology), Professor, Head of Department, Irkutsk State University, 1, K. Marx st., Irkutsk, 664003, Russian Federation, e-mail: vsalovarova@gmail.com

For citation
Makarova A.E., Yurinova G.V., Dzhioev Yu.P., Sukhov B.G., Pristavka A.A., Teterina G.A., Arefieva N.A., Borisenko A.Yu. Salovarova V.P., Evaluation of the Effects of Growth Stimulation of the Bifidobacterium with Polysaccharides from Siberian Larch. The Bulletin of Irkutsk State University. Series Biology. Ecology, 2023, vol. 43, pp. 66-74. https://doi.org/10.26516/2073-3372.2023.43.66 (in Russian)
Keywords
probiotic preparation Bifidobacterium bifidum, growth stimulation, polysaccharides, arabinogalactan, dihydroquercetin, quercetin, ardixin
UDC
577.127.4
DOI
https://doi.org/10.26516/2073-3372.2023.43.66
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