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«IZVESTIYA IRKUTSKOGO GOSUDARSTVENNOGO UNIVERSITETA». SERIYA «BIOLOGIYA. ECOLOGIYA»
«THE BULLETIN OF IRKUTSK STATE UNIVERSITY». SERIES «BIOLOGY. ECOLOGY»
ISSN 2073-3372 (Print)

List of issues > Series «Biology. Ecology». 2021. Vol 37

Comparison of the Respiration Process in Donor Winter Wheat Plants (Irkutskaya Variety) and Calli from this Variety in the Culture of Isolated Anthers

Author(s)
I. V. Lyubushkina, A. V. Pomortsev, M. S. Polyakova, G. A. Arbuzova, I. V. Ukolova, V. K. Voinikov, B. B. Anapiyaev
Abstract
Winter wheat is one of the main agricultural crops in the world. The constantly growing needs for food, as well as changing climatic conditions require modern breeding of new, more productive varieties with a high degree of cold, frost, drought and salt resistance,and resistance to pathogens and pests. Experimental haploidy can significantly reduce the time required for obtaining pure lines and new varieties. It is known that the physiological state of donor plants is very important for the effectiveness of androgenesis in vitro. In this work, we studied the features of flowers respiration in donor winter wheat plants used to isolate anthers and calli obtained in this culture. Winter wheat of Irkutskaya variety was used in the work. The plants were grown at the artificial climate station of Siberian Institute of Plant Physiology and Biochemistry SB RAS and in the field conditions of the Zalarinsky agroecological station. The plant material was selected at the booting stage. The initial medium was modified N6 containing sucrose (90 g/L), glycine (2 mg/L), 2,4-D (1 mg/L), and NAA (1 mg/L). The medium for regeneration was 190-2Cu with the addition of growth regulators 2,4-D (0.5 mg/L) and kinetin (0.5 mg/L). The oxygen uptake rate was measured at 26 ° C using an Oxyterm system (Han-satech Instruments, Great Britain). To determine the contribution of the main cytochrome and alternative cyanide-resistant pathways to respiration, an inhibitory assay was used. It was shown that opening of the flag leaf, as well as the procedure of anther isolation, led to an increase in the respiratory activity of winter wheat flowers, mainly due to the activation of the cytochrome pathway. At the same time, long-term low-temperature exposure contributed to the stabilization of respiration at the control level and did not cause changes in the activity of the alternative cyanide-resistant oxidase. The maximum respiratory activity was recorded in rhizogenic calli, while the respiratory activity of gemmorhizogenic calli was similar in respiration rate and contribution of the cytochrome and alternative pathways to the respiration of anther donor flowers.
About the Authors

Lyubushkina Irina Viktorovna, Candidate of Sciences (Biology), Research Scientist, Siberian Institute of Plant Physiology and Biochemistry SB RAS, 132, Lermontov st., Irkutsk, 664033, Russian Federation, Assistant Professor, Irkutsk State University, 1, Karl Marx st., Irkutsk, 664003,Russian Federation, e-mail:ostrov1873@yandex.ru

Pomortsev Anatolii Vladimirovich, Candidate of Science (Biology), Research Scientist Siberian Institute of Plant Physiology and Biochemistry SB RAS, 132, Lermontov st., Irkutsk, 664033, Russian Federation, e-mail: pomorcevanatolii@mail.ru

Polyakova Marina Stanislavovna, Lead Engineer, Siberian Institute of Plant Physiology and Biochemistry SB RAS, 132, Lermontov st., Irkutsk, 664033,Russian Federation, e-mail: poljakova.m@gmail.com

Arbuzova Galina Andreevna, Lead Engineer, Siberian Institute of Plant Physiology and Biochemistry SB RAS, 132, Lermontov st., Irkutsk, 664033, Russian Federation, Undergraduate, Irkutsk State Univercity, 1, Karl Marx st., Irkutsk, 664003,Russian Federation, e-mail: mingronland@mail.ru

Ukolova Irina Vladimirovna, Candidate of Science (Biology), Senior Researcher, Siberian Institute of Plant Physiology and Biochemistry SB RAS, 132, Lermontov st., Irkutsk, 664033, Russian Federation, e-mail: irina@sifibr.irk.ru

Voinikov Victor Kirillovich, Doctor of Sciences (Biology), Professor, Scientific Director, Siberian Institute of Plant Physiology and Biochemistry SB RAS, 132, Lermontov st., Irkutsk, 664033, Russian Federation, e-mail: vvk@sifibr.irk.ru

Anapiyaev Bahytzhan Beisenbekovich, Doctor of Sciences (Biology), Satbayev University, 22a, Satpaev st., Almaty, 050013, Kazakhstan, e-mail: bak_anapiyayev@mail.ru

For citation
Lyubushkina I.V., Pomortsev A.V., Polyakova M.S., Arbuzova G.A., Ukolova I.V., Voinikov V.K., Anapiyaev B.B. Comparison of the Respiration Process in Donor Winter Wheat Plants (Irkutskaya Variety) and Calli from this Variety in the Culture of Isolated Anthers. The Bulletin of Irkutsk State University. Series Biology. Ecology, 2021, vol. 37, pp. 3-15. https://doi.org/10.26516/2073-3372.2021.37.3 (in Russian)
Keywords
winter wheat, isolated anther culture, induced androgenesis, morphogenic and non-morphogenic calli, respiration, cytochrome and alternaive pathways.
UDC
57.085.23
DOI
https://doi.org/10.26516/2073-3372.2021.37.3
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