Due to the unique chemical composition, the consumption of the highest edible shiitake mushroom has a beneficial effect on the state of many systems of the body and helps to significantly improve human health. Various pharmacological forms of the fungus have excellent antimicrobial properties at the same time, without disturbing the life of microorganisms from the normal human microflora. Mushroom juice extract has excellent antimicrobial and antifungal properties, while not disrupting the life of the normal intestinal microflora of humans. Maintaining the quasi-static state of the main groups of representatives of normal microflora (E. coli, bifidobacteria and lactobacilli) is a very promising task to ensure the necessary physiological processes of the body. The study used Lactobacillus acidophilus from lyophilized preparation “Lactobacterin” and lactobacterial complex autoprobiotics received from specific people. As a growth stimulant of lactobacilli, the juice or powder of shiitake mushroom (Lentinus edodes) was used in an amount of 2.5 % of the nutrient medium. To assess the development of populations of cells of lactobacilli we used nephelometric method of the study and the method of direct count of colony forming units (CFU) on plates with agar nutrient dense environment (lactobacilus). As a result of this work, the stimulation of lactobacilli contained in the drug "lactobacilli"was established. The greatest effect was obtained when adding Shiitake mushroom powder to a dense medium-an increase in CFU by 46.2% relative to the control was found. Another drug-juice of Shiitake mushroom promotes active growth of lactobacilli complex autoprobiotics, judging by the increase in optical density 2.5 times as nephelometric valuation method. During the work it was proved a stimulating effect of pharmacological forms of Shiitake on the development of Lactobacillus, the probiotic preparation "Lactobacterin", and also because of autoprobiotics people. The results suggest that the addition of shiitake mushroom juice or powder significantly increases the therapeutic effect of any probiotic drug by increasing the viability of lactobacilli. Shiitake mushroom derivatives (Lentinus edodes) can be used as natural stimulants for the development of lactobacilli to develop new probiotic drugs to correct intestinal dysbiosis or create functional foods.

Kirilenko Marina Aleksandrovna, Senior Lecturer, Ivanovo State Medical Academy, 8, Sheremetevski Av., Ivanovo, 153012, Russian Federation, е-mail: smarina23@mail.ru 

Kuznetsov Oleg Yuvenal'evich, Doctor of Sciences (Biology), Professor, Head of Chair, Ivanovo State Medical Academy, 8, Sheremetevski Av., Ivanovo, 153012, Russian Federation, е-mail: olegkuz58@ya.ru

Kirilenko M.A., Kuznetsov O.Yu. Assessment of Biological Effects of Pharmacological Forms of Shiitake (Lentinus edodes) on Lactobacilli. The Bulletin of Irkutsk State University. Series Biology. Ecology, 2019, vol. 28, pp. 56–62. https://doi.org/10.26516/2073-3372.2019.28.56 (in Russian)

microbiology, Lactobacillus, Shiitake mushroom, juice, powder, complex autoprobiotics

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Kuznetsov O.Yu., Sotnikova N.Yu., Martenova A.A., Kulikova E.V. Ingibiruyushchee deistvie soka griba Shiitake na patogennye i uslovno-patogennye mikroorganizmy [Inhibitory effect of Shiitake juice on opportunistic and pathogenic microorganisms]. Aktualnye problemy zdorov'ya sem'i [Actual problems of family health]. Ivanovo, 2000, p. 193.

Kirilenko M.A., Kuznetsov O.Yu. Sposob polucheniya preparata eubiotika Laktobakterin [Method for producing of eubiotic preparate Laktobakterin]. Rus. Fed. Pat. 2661737 RF. MPK51 A61K 35/74. Publ. 19.07.2018. Bul. no. 20, 4 p.

Yanovoi V.V., Martynov A.S., Razin A.N., Filippova I.F. Primenenie gribov Lentinus edodes (Shiitake) v kompleksnom lechenii zapushchennykh form raka obodochnoi i pryamoi kishki [Use of Shiitake in complex treatment of terminal colorectal cancer]. Koloproktologiya, 2006, no. 1 (15), pp. 32-34.

Kuznetsov O.Yu., Kuznetsova L.A., Kuznetsov A.O., Borisova E.M., Safonova M.A. Sposob polucheniya autoprobiotika, soderzhashchego zhivye laktobakterii i bifidobakterii [Method for producing of probiotic contained live lactic acid bacteria and bifidobacteria]. Rus. Fed. Pat. 2505304 RF; MPK51 A61K 35/74, A23C 9/127. Publ. 27.01.2014. Bul. no. 3, 7 p.

Hobbs C. Medicinal Value of Lentinus edodes (Berk.) Sing. (Agaricomycetideae). A Literature Review. Int. J. Med. Mushr., 2000, vol.2, no. 4, pp. 287-302. https://doi.org/10.1615/IntJMedMushr.v2.i4.90 

Lelley J.I., Vetter J. Orthomolecular medicine and mushroom consumption: An attractive aspect for promoting production. Science and Cultivation of Edible and Medicinal Fungi. Romaine, Keil, Rinker, Royse (Eds.). Pennsylvania St. Univ., 2004, pp. 637-643.

Sano M., Yoshino K., Matsuzawa T. Inhibitory effects of edible higher basidiomycetes mushroom extracts on mouse type IV allergy. Int. J. Med. Mushr., 2002, vol. 4, no. 1, pp. 37-41. https://doi.org/10.1615/IntJMedMushr.v4.i1.40

Sia G.M., Candlish J.K. Effect of shiitake (Lentinus edodes) extract on human neutrophils and the monocytic cell line. Phytotherapy Research, 1999, vol.13, no. (2), pp. 133-137. https://doi.org/10.1002/(SICI)1099-1573(199903)13:2<133::AID-PTR398>3.0.CO;2-O

Stamets P. Potentiation of cell-mediated host defense using fruitbodies and mycelia of medicinal mushrooms. Int. J. Med. Mushr., 2003, vol. 5, no. 2, pp. 179-192. https://doi.org/10.1615/InterJMedicMush.v5.i2.70