In order to elucidate the phylogenetic relationships within Alticola voles mitochondrial cytochrome b (cyt b) gene variation was estimated in 5 Alticola species. Maximum likelihood (ML) and Bayesian phylogenetic analysis were used for the reconstruction of phylogenetic relationships. The results are substantially complete the picture of the phylogenetic relationships in the genus Alticola by the inclusion of Tyva vole A. tuvinicus and Olkhon vole A. olchonensis in the analysis. It is shown that A. olchonensis is a valid species, which is associated with Mongolian silver vole A. semicanus and A. tuvinicus in closely related species group sister to the flatheaded vole A. strelzowi.