The paper discusses the problem of obtaining tissue cultures of Abies sibirica Ledeb. in vitro. The vegetative shoots taken from aged trees were used as primary explants growing in Irkutsk and 70 km apart, in the countryside. Chosen were (i) the conditions of sterilization of shoot apexes, (ii) the components of nutrient media to obtain tissue cultures. The explant type was chosen. Using the apical buds of the sprouts and the nutrient media characterized by varied proportions of growth regulators, we managed to optimize the growth and development conditions for the sprouts and callusogenesis induction during the spring-summer season. The results obtained may be applied in further experiments, which presume obtaining morphogenic tissue cultures of Abies sibirica Ledeb., and also in the process of elaboration of the conditions for microclonal reproduction of valuable decorative forms of conifer trees. This elaboration was conducted to the end of landscaping and reafforestation.