Biofilm formation with bacteria Escherichia coli (strain XL1-Blue) and Pectobacterium carotovorum (strain B-1247) on the surface of polystyrene plates have been studied. The dynamics of using the accumulation of biofilm tablet method for buffered saline, buffered saline, with the content of 0,1 % glucose and 0,5 % glucose solution, was independent of the original amount of bacterial cells, depending on the composition of the medium, in particular, making sugars. Introducing into the culture medium in higher glucose concentration promotes process bio-film formation that was recorded on the surface of polystyrene plates. Results indicate that the mechanism of biofilm formation is similar to the bacteria that live in different ecological niches (Pectobacterium carotovorum strain B-1247 is a necrotrophic phytopathogen type of nutrient metabolism, and Escherichia coli strain XL1-Blue - it is conditional-pathogenic microorganism for human, widespread in the environment), but they have apparently similar mechanisms of biofilm formation.