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«IZVESTIYA IRKUTSKOGO GOSUDARSTVENNOGO UNIVERSITETA». SERIYA «BIOLOGIYA. ECOLOGIYA»
«THE BULLETIN OF IRKUTSK STATE UNIVERSITY». SERIES «BIOLOGY. ECOLOGY»
ISSN 2073-3372 (Print)

List of issues > Series «Biology. Ecology». 2025. Vol 54

Development and Evaluation of the Efficiency of a Device for Filtration of Nutrient Media

Author(s)

S. V. Lukyanova, V. Yu. Mikloshevich, N. G. Gefan, V. S. Kosilko, V. I. Kuznetsov

Irkutsk Research Anti-Plague Institute of Siberia and Far East by Rospotrebnadzor, Irkutsk, Russian Federation

Abstract
Ready-made nutrient media are used in microbiology for isolation, cultivation of microorganisms, their storage and transportation, as well as for studying cultural, biochemical, antigenic properties, phagolizability and sensitivity to antibiotics. Practically all nutrient media of domestic production need to be filtered before the sterilization stage, according to the manufacturer's instructions. The design features of the apparatus for the preparation of microbiological nutrient media, combined with a filling module, exclude the filtration stage and require additional equipment with an external filtering device. A device for filtration of agar nutrient media has been developed and tested, which can be used directly in the apparatus for the preparation of microbiological nutrient media and subsequent sterile filling into Petri dishes using a filling module. The filter housing is installed inside the device for preparing nutrient media, equipped with a lid with a lock, hygroscopic white cotton wool is used as the filter material. Filtration is carried out by means of external pressure from the supply side of the filtered liquid. The proposed device makes it possible to clarify the nutrient medium by delaying solid phase particles, reduces operating time and provides safer working conditions for personnel.
About the Authors

Luk'yanova Svetlana Vladimirovna, Candidate of Sciences (Biology), Research Scientist Irkutsk Research Anti-Plague Institute of Siberia and Far East by Rospotrebnadzor 78, Trilisser st., Irkutsk, 664047, Russian Federation e-mail: svetalukyan@mail.ru 

Mikloshevich Vitaly Yurievich, Process Engineer Irkutsk Research Anti-Plague Institute of Siberia and Far East by Rospotrebnadzor 78, Trilisser st., Irkutsk, 664047, 98 Russian Federation e-mail: adm@chumin.irkutsk.ru 

Gefan Natalya Gennadyevna, Candidate of Sciences (Medicine), Senior Research Scientist Irkutsk Research Anti-Plague Institute of Siberia and Far East by Rospotrebnadzor 78, Trilisser st., Irkutsk, 664047, Russian Federation e-mail: adm@chumin.irkutsk.ru 

Kosilko Varvara Sergeevna, Physician Bacteriologist Irkutsk Research Anti-Plague Institute of Siberia and Far East by Rospotrebnadzor 78, Trilisser st., Irkutsk, 664047, Russian Federation e-mail: adm@chumin.irkutsk.ru 

Kuznetsov Vladimir Ilyich, Candidate of Sciences (Biology), Head of Laboratory Irkutsk Research Anti-Plague Institute of Siberia and Far East by Rospotrebnadzor 78, Trilisser st., Irkutsk, 664047

For citation
Lukyanova S.V., Mikloshevich V.Yu., Gefan N.G., Kosilko V.S., Kuznetsov V.I. Development and Evaluation of the Efficiency of a Device for Filtration of Nutrient Media. The Bulletin of Irkutsk State University. Series Biology. Ecology, 2025, vol. 54, pp. 92-98. https://doi.org/10.26516/2073-3372.2025.54.92 (in Russian)
Keywords
cultivation of microorganisms, nutrient medium, filtration.
UDC
608.2
DOI
https://doi.org/10.26516/2073-3372.2025.54.92
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