The CLAVATA3 gene encodes a peptide consisting of 78 amino acids, capable of transport through the apoplast, while in the mature state it is glycosylated with L-arabinose and consists of 12 amino acids that make up the so-called CLE domain. This peptide in the apical meristem of the shoot blocks cell proliferation and activates cell differentiation. We have previously created and studied transgenic tobacco plants with constitutive expression of the CLAVATA3 Arabidopsis thaliana gene. All the analyzed transgenic plants were characterized by a decrease in the number of cells in the shoot organs compared to the wild type, but most of the transgenic plants did not differ from the control by phenotype due to compensatory cell growth. However, the features of root growth of tobacco plants transgenic by the CLAVATA3 gene remained unknown. Therefore, the purpose of this work was to study the root growth of tobacco plants transgenic by the CLAVATA3 gene under normal © Бережнева З. А., Кулуев Б. Р., 2025 *Полные сведения об авторах см. на последней странице статьи. For complete information about the authors, see the last page of the article. РОСТ КОРНЕЙ ТРАНСГЕННЫХ РАСТЕНИЙ ТАБАКА С ЭКСПРЕССИЕЙ ГЕНА CLAVATA3 81 and stressful conditions. In vitro, the growth of roots of transgenic tobacco plants 35S::CLAVATA3 was determined on vertically oriented Petri dishes under normal conditions and under the action of stress factors: salinity (50 and 100 mM NaCl), cadmium (100, 200 and 400 mkM CdAc), hypothermia +10°C. Transgenic tobacco plants were characterized by improved root growth under normal conditions, the effect of salinization and cadmium ions compared to the wild type. In hypothermia, the growth rates of the roots of transgenic plants did not significantly differ from the wild type. In our previous studies, it was shown that overexpression of the CLAVATA3 gene promotes the stimulation of cell expansion in the shoot organs. In the roots of 35S:: CLAVATA3 plants, as well as in the shoot organs, cell expansion was stimulated, which ultimately resulted in improved root growth, both under normal conditions and under the action of stress factors. The CLAVATA3 transgen can be used to improve root growth under normal conditions, the action of salinization and cadmium ions.

Berezhneva Z.A., Kuluev B.R. Root Growth of Transgenic Tobacco Plants with Constitutive Expression of the CLAVATA3 Gene under the Action of Stress Factors. The Bulletin of Irkutsk State University. Series Biology. Ecology, 2025, vol. 53, pp. 80-86. https://doi.org/10.26516/2073-3372.2025.53.80 (in Russian)

Nicotiana tabacum, transgenic plants, root growth, salinity, hypothermia, cadmium

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