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«IZVESTIYA IRKUTSKOGO GOSUDARSTVENNOGO UNIVERSITETA». SERIYA «BIOLOGIYA. ECOLOGIYA»
«THE BULLETIN OF IRKUTSK STATE UNIVERSITY». SERIES «BIOLOGY. ECOLOGY»
ISSN 2073-3372 (Print)

List of issues > Series «Biology. Ecology». 2025. Vol 52

Hydrochemical Conditions and Composition of the Bacterial Community in Lake Ak-Khol (Tyva Republic)

Author(s)

E. G. Tokmakova1, O. I. Kal’naya2, S. V. Erdyneev1, A. S. Ponomaryova1, N. F. Galatsevich3

1 Irkutsk Research Anti-Plague Institute by Rospotrebnadzor, Irkutsk, Russian Federation

2 Tuva Institute of Integrated Development of Natural Resources SB RAS, Kyzyl, Russian Federation

3 Tyva Anti-Plague Station by Rospotrebnadzor, Kyzyl, Russian Federation

Abstract
Lake Ak-Khol is located in Mongun-Taiga district of Tyva Republic, bounded by coordinates N50º23′, E090º16′and has economic, environmental and recreational significance. Chemical properties of water and total DNA of bacterial community from the coastal zone of the lake were analyzed. For chemical analysis, water was sampled at four points (east, south, southwest, north), and for metagenomic analysis, samples of water and silt were collected at one point near the eastern shore of the lake. The dissolved oxygen content of the water was 7.40-7.86 mg/litre. The lake waters are predominantly oligosaline with mineralisation of 1.471–1.514 g/l, except for the south-western part of the lake, where mineralisation decreases to 0.736 g/l due to groundwater discharge in the form of a spring with a total flow rate of 4.5 l/s.The ionic composition is formed mainly by sodium hydrogen carbonate and sodium chloride, pH of the medium is alkaline (8.5–8.8), hardness is mild (1.60–2.80 mg-eq/l).No petroleum products were detected. Phenols were present in only one sample (east shore). Concentrations of anionic surfactants ranged from 0 (south-west shore) to 0.04 mg/l (south shore). The content of heavy metals, mercury and halogens did not exceed the maximum permissible concentrations established by the state norms 2.1.5.1315-03 for water of water bodies for household and cultural water use. According to the results of metagenomic analysis, Bacteroidota phyla (46.58 % of sequences), genus Candidatus Aquirestis (46.58 %), was dominant in the water, while Actinobacteria phyla (52.73 %), genus Nitriliruptor (35.40 %), was dominant in the silt. Representatives of the families Rhodobacteraceae (except genus Paracoccus), Sphingomonadaceae and Rubritaleaceae were common for the microbiota of water and silt, accounted for 24.91 % in water and 18.8 % in silt. More than half of them in both cases was provided by the presence of YooniaLoktanella halophile sequences – 14.53 % in water and 10.09 % in silt. It is concluded that further studies of the lake are necessary.
About the Authors

Tokmakova Elena Gennadyevna, Candidate of Sciences (Biology), Senior Research Scientist Anti-Plague Research Institute of Siberia and Far East of Rospotrebnadzor 78, Trilisser st., Irkutsk, 664047, Russian Federation e-mail: flea98@mail.ru

Kal’naya Ol’ga Ivanovna, Candidate of Sciences (Geography), Senior Research Scientist Tuva Institute of Integrated Development of Natural Resources SB RAS 117A, Internatsionalnaya st., Kyzyl, 667007, Russian Federation e-mail: kalnaja@mail.ru

Erdyneev Sergey Viktorovich, JuniorResearch Scientist Irkutsk Anti-Plague Research Institute of Siberia and Far East of Rospotrebnadzor 78, Trilisser st., Irkutsk, 664047, Russian Federation e-mail: orry230@yandex.ru

Ponomaryova Anna Sergeevna, Bacteriologist Irkutsk Anti-Plague Research Institute of Siberia and Far East of Rospotrebnadzor 78, Trilisser st., Irkutsk, 664047, Russian Federation e-mail: ackozh@mail.ru

Galatsevich Nina Feliksovna, Zoologist Tyva Anti-Plague Station by Rospotrebnadzor 13, Moskovskaya st., Kyzyl, 667010, Russian Federation e-mail: nf-gala@mail.ru

For citation
Tokmakova E.G., Kal’naya O.I., Erdyneev S.V., Ponomaryova A.S., Galatsevich N.F. Hydrochemical Conditions and Composition of the Bacterial Community in Lake Ak-Khol (Tyva Republic). The Bulletin of Irkutsk State University. Series Biology. Ecology, 2025, vol. 52, pp. 87-95. https://doi.org/10.26516/2073-3372.2025.52.87 (in Russian)
Keywords
Republic of Tyva, Ak-Khol Lake, oligosaline lakes, chemical analysis of water, bacterial community.
UDC
543.32/.34+574.4726:579.26
DOI
https://doi.org/10.26516/2073-3372.2025.52.87
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