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«IZVESTIYA IRKUTSKOGO GOSUDARSTVENNOGO UNIVERSITETA». SERIYA «BIOLOGIYA. ECOLOGIYA»
«THE BULLETIN OF IRKUTSK STATE UNIVERSITY». SERIES «BIOLOGY. ECOLOGY»
ISSN 2073-3372 (Print)

List of issues > Series «Biology. Ecology». 2025. Vol 51

Growth-Stimulating Activity of Pseudomonas sp. Strain GEOT18 from the Tuberoid Orchid Dactylorhiza incarnata

Author(s)

I. I. Rassokhina1, O. A. Marakaev2, A. V. Platonov1,3

1 Vologda Research Center RAS, Vologda, Russian Federation 

2 P. G. Demidov Yaroslavl State University, Yaroslavl, Russian Federation 

3 Vologda Institute of Law and Economics of the Federal Penitentiary Service of Russia, Vologda, Russian Federation

Abstract
Currently, microorganisms that can increase agricultural crop yields, particularly bacteria of the Pseudomonas genus, are generating interest as an alternative to chemical intensification agents in agricultural production. The growth-promoting effect of these bacteria is associated with the synthesis of various metabolites, which can possess signaling and phytohormonal activity, increase nutrient availability for plants, stimulate systemic resistance, or suppress the development of phytopathogens. The aim of our work was to evaluate the possibility of using a suspension of Pseudomonas sp. GEOT18 strain, isolated from the tuberoids of Dactylorhiza incarnata (L.) Soó (Orchidaceae), for cultivating Hordeum vulgare L. The study included an evaluation of the bacterial colonization potential (laboratory trial) and its effect on the growth and productivity (field trials) of barley variety Sonet. The laboratory trial was carried out by soil culture method, the evaluation of colonization capacity included the search for PA-GS and PsEG genes in barley rhizosphere samples. For the control in the laboratory study, a cell culture of the studied strain grown on Miller's LB medium was used. In the field experiment, the bacterial suspension was applied twice: through pre-sowing seed soaking and by spraying the phyllosphere during the tillering phase. We determined the morphophysiological and production parameters of barley during the growing season. At the beginning of the waxy ripeness stage, the final grain yield of the experimental and control plants was determined, and the yield structure was assessed (number of kernels per ear and the weight of 1,000 kernels). The results of the laboratory experiment demonstrate the presence of the PA-GS and PsEG genes both in the pure GEOT18 culture sample and in the barley rhizosphere sample 11 days after seed inoculation and planting. This indicates the ability of the studied bacteria to successfully colonize the barley root system after pre-sowing seed treatment. The field research shows that Pseudomonas sp. GEOT18 strain contributes to an increase in leaf surface area, photosynthetic pigment content, dry weight, and net photosynthetic productivity of barley. For instance, the dry weight of the Sonet barley variety increased by 29–64 % at the heading stage and by 70–88 % at the flowering stage following the application of the bacterial suspension. Grain productivity of the experimental variants of H. vulgare variety Sonet exceeded the control by 20–40 %. It can be assumed that a preparation based on this strain will be highly effective, considering that the studied bacteria are able to establish themselves in the barley rhizosphere.
About the Authors

Rassokhina Irina Igorevna, Research Scientist Vologda Research Center RAS 56A, Gorky st., Vologda, 160014, Russian Federation e-mail: rasskhinairina@mail.ru

Marakaev Oleg Anatolyevich, Candidate of Science (Biology), Associate Professor P. G. Demidov Yaroslavl State University 14, Sovetskaya st., Yaroslavl, 150003, Russian Federation e-mail: marakaev@uniyar.ac.ru 

Platonov Andrei Viktorovich, Candidate of Science (Biology), Leading Research Scientist Vologda Research Center RAS 56A, Gorky st., Vologda, 160014, Russian Federation Associate Professor Vologda Institute of Law and Economics of the Federal Penitentiary Service of Russia 2, Schetinin st., Vologda, 160002, Russian Federation e-mail: platonov70@yandex.ru

For citation
Rassokhina I.I., Marakaev O.A., Platonov A.V. Growth-Stimulating Activity of Pseudomonas sp. Strain GEOT18 from the Tu-beroid Orchid Dactylorhiza incarnata. The Bulletin of Irkutsk State University. Series Biology. Ecology, 2025, vol. 51, pp. 3-15. https://doi.org/10.26516/2073-3372.2025.51.3 (in Russian)
Keywords
Hordeum vulgare, Pseudomonas, strain, growth, productivity, photosynthetic pigments.
UDC
581.14+579.64+633.16
DOI
https://doi.org/10.26516/2073-3372.2025.51.3
References
  1. Levakova O.V., Dedushev I.A., Yeroshenko L.M., Romakhin M.M., Yeroshenko A.N., Yeroshenko N.A., Boldyrev M.A., Gladysheva O.V. Vliyanie agrometeorologicheskikh izmenenii klimata na zernovuyu produktivnost' yarovogo yachmenya v usloviyakh Nechernozemnoi zony RF [The influence of agrometeorological climate changes on the grain productivity of spring barley in the conditions of the Non-Chernozem Zone of the Russian Federation]. Yug Rossii: ekologiya, razvitiye [South of Russia: Ecology, Development], 2022, no. 17 (62), pp. 128-135. (in Russian) 
  2. Gavrilenko V.F., Zhigalova T.V. Bolshoy praktikum po fotosintezu [Large workshop on photosynthesis]. Moscow, Akademiya Publ., 2003, 256 p. (in Russian) 
  3. Dospekhov B.A. Metodika polevogo opyta (s osnovami statisticheskoy obrabotki rezultatov issledovaniya) [Field experience methodology (with the basics of statistical processing of research results)]. Moscow, Alyans Publ., 2011, 352 p. (in Russian) 
  4. Ivanov V.A. Strategiya razvitiya selskogo khozyaystva Yevropeyskogo Severa Rossii [Strategy for the development of agriculture in the European North of Russia]. Syktyvkar, Print Publ., 2023, 140 p. (in Russian) 
  5. Maksimov I.V., Abizgildina R.R., Pusenkova L.I. Stimuliruyushhie rost rastenij mikroorganizmy kak alternativa ximicheskim sredstvam zashhity ot patogenov [Microorganisms that stimulate plant growth as an alternative to chemical means of protection against pathogens.]. Applied Biochemistry and Microbiology, 2011, vol. 47, no. 4, pp. 373-385. (in Russian) 
  6. Nichiporovich A.A. Fotosintez i teoriya polucheniya vysokikh urozhayev [Photosynthesis and the theory of obtaining high yields]. Moscow, AS USSR Publ., 1956, 94 p. (in Russian) 
  7. Rassokhina I.I., Platonov A.V. Deystviye bakteriy roda Pseudomonas sp. na biologicheskuyu i khozyaystvennuyu produktivnost pshenitsymyagkoy v usloviyakh Vologodskoy oblasti [The effect of bacteria of the genus Pseudomonas sp. on the biological and economic productivity of soft wheat in the conditions of the Vologda region]. Int. Agric. J., 2021, vol. 64, no. 5, pp. 37-49. (in Russian) 
  8. Rassokhina I.I., Platonov A.V., Platonov А.А. Deystviye bakteriy roda Pseudomonas sp. na rostiproduktivnost' triticale [Effect of bacteria of the genus Pseudomonas sp. on the growth and productivity of triticale]. Bulletin of KSAU, 2022, no. 1(178), pp. 93-99. (in Russian) https://doi.org/10.36718/1819-4036-2022-1-93-99
  9. Titova M.S. Soderzhaniye fotosinteticheskikh pigmentov v khvoye Picea abies i Picea koraiensis [Content of photosynthetic pigments in the needles of Picea abies and Picea koraiensis]. Vestnik Orenburg St. Univ., 2010, no. 12, pp. 9-12. (in Russian) 
  10. Bychkova A.A., Zaitseva Y.V., Sidorov A.V., Aleksandrova A.S., Marakaev O.A. Biotechnological potential fphosphate-solubilizing Pseudomonas migulae strain GEOT18. IJAT, 2022, vol. 18, no. 4, pp. 1403-1414. 
  11. Lazarus H.P.S., Easwaran N. Molecular insights into PGPR fluorescent Pseudomonads complex mediated intercellular and interkingdom signal transduction mechanisms in promoting plant's immunity. Res Microbiol., 2024, vol. 175, is. 7, 104218. https://doi.org/10.1016/j.resmic.2024.104218 
  12. Mankoti M., Pandit N.K., Meena S.S., Mohanty A. Investigating the genomic and metabolic abilities of PGPR Pseudomonas fluorescens in promoting plant growth and fire blight management. Mol. Genet. Genomics, 2024, no. 299, 110. https://doi.org/10.1007/s00438-024-02198-3 
  13. Kumari P., Meena M., Gupta P., Dubey M.K., Nath G., Upadhyay R. Plant growth promoting rhizobacteria and their biopriming for growth promotion in mung bean (Vigna radiata (L.) R. Wilczek). Biocatal. Agric. Biotechnol., 2018, vol. 16, pp. 163-171. https://doi.org/10.1016/j.bcab.2018.07.030
  14. Choi O., Kim J., Kim J.G., Jeong Y., Moon J.S., Park C.S., Hwang I. Pyrroloquinoline quinone is a plant growth promotion factor produced by Pseudomonas fluorescens B16. Plant Physiol., 2008, vol. 146, is. 2, pp. 657-668. https://doi.org/10.1104/pp.107.112748 
  15. Sah S., Krishnani S., Singh R. Pseudomonas mediated nutritional and growth promotional activities for sustainable food security. Curr. Res. Microb. Sci., 2021, vol. 2, 100084. https://doi.org/10.1016/j.crmicr.2021.100084 
  16. Sun W., Shahrajabian M. H., Soleymani A. The Roles of Plant-Growth-Promoting Rhizobacteria (PGPR)-Based Biostimulants for Agricultural Production Systems. Plants, 2024, vol. 13, is. 5, 613. https://doi.org/10.3390/plants13050613 
  17. Singh P., Singh R.K., Guo D.-J., Sharma A., Singh R.N., Li D.-P., Malviya M.K., Song X.-P., Lakshmanan P., Yang L.-T., Li Y.-R. Whole genome analysis of sugarcane root-associated endophyte Pseudomonas aeruginosa B18-A plant growth-promoting bacterium with antagonistic potential against Sporisoriums citamineum. Front. Microbiol., 2021, vol. 12, 628376. https://doi.org/10.3389/fmicb.2021.628376



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