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«IZVESTIYA IRKUTSKOGO GOSUDARSTVENNOGO UNIVERSITETA». SERIYA «BIOLOGIYA. ECOLOGIYA»
«THE BULLETIN OF IRKUTSK STATE UNIVERSITY». SERIES «BIOLOGY. ECOLOGY»
ISSN 2073-3372 (Print)

List of issues > Series «Biology. Ecology». 2024. Vol 47

Inhibitory Analysis of Protease Preparations of Cell-Free Fractions of Cholera Vibrios

Author(s)
S. N. Kozlov, E. Yu. Markov, V. B. Nikolaev, L. V. Mironova, A. V. Korneva, A. A. Doroshchenko
Abstract
One of the important areas of research into the pathogenesis of diseases caused by pathogens of especially dangerous infections is the study of the system of hydrolytic enzymes. It has been established that hydrolytic enzymes of pathogenic microorganisms enhance their pathogenicity (virulence) and participate in the processes of adaptation to unfavourable environmental factors. Cholera vibrio has previously electrophoretically detected hydrolases belonging to different subclasses and established differences in their spectrum in strains with different epidemic significance. Therefore, the study of their characterisation (type determination using activator/inhibitor assays) is a relevant area of research. The inhibitor analysis of secreted proteases of cholera vibrio strains of O1 serogroup El Tor biovar and O139 serogroup determined that the proteases include serine and metalloproteases.
About the Authors

Kozlov Stanislav Nikolaevich, Research Scientist, Irkutsk Anti-Plague Research Institute of Siberia and Far East of Rospotrebnadzor, 78, Trilisser st., Irkutsk, 664047, Russian Federation, e-mail: ejimei@mail.ru

Markov Evgeniy Yurievich, Doctor of Sciences (Biology), Senior Research Scientist, Irkutsk Anti-Plague Research Institute of Siberia and Far East of Rospotrebnadzor, 78, Trilisser st., Irkutsk, 664047, Russian Federation, e-mail: mark_evgenii@mail.ru

Nikolaev Valeriy Borisovich, Candidate of Sciences (Medicine), Senior Research Scientist, Irkutsk Anti-Plague Research Institute of Siberia and Far East of Rospotrebnadzor, 78, Trilisser st., Irkutsk, 664047, Russian Federation, e-mail: eaverschinin@mail.ru

Mironova Lilya Valerievna, Doctor of Sciences (Medicine), Deputy Director Irkutsk Anti-Plague Research Institute of Siberia and Far East of Rospotrebnadzor, 78, Trilisser st., Irkutsk, 664047, Russian Federation, e-mail: mironova-lv@yandex.ru

Korneva Aleksandra Vladimirovna, Junior Research Scientist, Irkutsk Anti-Plague Research Institute of Siberia and Far East of Rospotrebnadzor, 78, Trilisser st., Irkutsk, 664047, Russian Federation, e-mail: korneva@inbox.mail.ru

Doroschenko Anton Aleksandrovich, Junior Research Scientist, Irkutsk Anti-Plague Research Institute of Siberia and Far East of Rospotrebnadzor, 78, Trilisser st., Irkutsk, 664047, Russian Federation, e-mail: yashik.genry@mail.ru

For citation

Kozlov S.N., Markov E.Yu., Nikolaev V.B., Mironova L.V., Korneva A.V., Doroshchenko A.A. Inhibitory Analysis of Protease Preparations of Cell-Free Fractions of Cholera Vibrios. The Bulletin of Irkutsk State University. Series Biology. Ecology, 2024, vol. 47, pp. 64-70. https://doi.org/10.26516/2073-3372.2024.47.64 (in Russian)

Keywords
zymography, polyacrylamide gel, cell-free extracts, Vibrio cholerae, inhibitors, activators.
UDC
616.932(471)+57.579
DOI
https://doi.org/10.26516/2073-3372.2024.47.64
References

Kozlov S.N., Markov E.Yu., Nikolaev V.B., Mironova L.V., Mitkeeva S.K., Urbanovich L.Ya. Zimograficheskaya detektsiya gidrolaz Vibrio cholerae O1 i O139 serogrupp s ispol'zovaniem spetsificheskikh substratov [Zymographic detection of Vibrio cholerae O1 and O139 serogroups hydrolases using specific substrates]. Int. Res. J., 2017, no.11, pp. 70-76. https://doi.org/10.23670/IRJ.2017.65.131. (in Russian)

Fierer J., Looney D., Pechère J. C. Nature and pathogenicity of microorganisms. Infect. Dis., 2017, vol. 1, pp. 4-25. e1. https://doi.org/10.1016/B978-0-7020-6285-8.00002-2

Ma L., Wu J., Zheng Y., Shu Z., Wei Z., Sun Y., Carrell R.W., Zhou A. Heparin blocks the inhibition of tissue kallikrein 1 by kallistatin through electrostatic repulsion. Biomolecules, 2020, vol. 10, no. 6, p. 828. https://doi.org/10.3390/biom10060828

Litov L., Petkov P., Rangelov M., Ilieva N., Lilkova E., Todorova N., Krachmarova E., Malinova K., Gospodinov A., Hristova R., Ivanov I., Nacheva G. Molecular mechanism of the antiinflammatory action of heparin. Int. J. Mol. Sci., 2021, vol. 22, no. 19, e10730. https://doi.org/10.3390/ijms221910730

Stewart-Tull D.E.S., Bleakley C.R., Galloway T.S. Characteristics of Vibrio cholerae proteinases: potential, candidate vaccine antigens. Vaccine, 2004, vol. 22, is. 23-24, pp. 3026-3034. https://doi.org/10.1016/j.vaccine.2004.02.012

Montero D.A., Vidal R.M., Velasco J., George S., Lucero Y., Gómez L.A., Carreño L.J., García-Betancourt R., O’Ryan M. Vibrio cholerae classification, pathogenesis, immune response, and trends in vaccine development. Front. Med., 2023, vol. 10. https://doi.org/10.3389/fmed.2023.1155751

Yu W.H., Woessner J.F. Heparin-enhanced zymographic detection of matrilysin and collagenases. Anal. Biochem., 2001, vol. 293, is. 1, pp. 38-42. https://doi.org/10.1006/abio.2001.5099


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