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«IZVESTIYA IRKUTSKOGO GOSUDARSTVENNOGO UNIVERSITETA». SERIYA «BIOLOGIYA. ECOLOGIYA»
«THE BULLETIN OF IRKUTSK STATE UNIVERSITY». SERIES «BIOLOGY. ECOLOGY»
ISSN 2073-3372 (Print)

List of issues > Series «Biology. Ecology». 2023. Vol 46

Baikalian Highway as a High-Risk Area for Transmissible Tick-Borne Infections

Author(s)
O. V. Mel’nikova, R. V. Adel’shin, E. A. Vershinin, K. V. Lopatovskaya, Yu. N. Trushina, E. I. Andaev
Abstract
The results of long-term monitoring of the taiga ticke I. persulcatus abundance and its infection rate with tick-borne encephalitis virus and bacteria of genus Anaplasma, Borrelia, Ehrlichia and Rickettsia in forests along the Baikalian highway (road No 25К-011 Irkutsk region, Russia). The ticks’ abundance varied in space and time from complete lack on the registration route up to 339 ticks per flag-hour. The dynamics during study period (2005-2022) had three waves of ups and downs. The maximum of long-term average seasonal activity occurred at second and third decade of May. The average numbers of ticks increased as distance from the city grew. The analysis with the help of multiplex test system for four infections (tick-borne encephalitis virus, Anaplasma, Borrelia, Ehrlichia) showed marker at least of one pathogen in 57 % of specimens, different mixes of two and more co-infections – in 16.3 %. From 10 variants of co-infections found in this sample, the most prevalent were combinations of Borrelia, Ehrlichia and Anaplasma (74.7 % of double and 7.5 % triple «mixes»). Least often, the ticks were co-infected with tick-borne encephalitis virus in combination with Ehrlichia and Anaplasma (0.5 % each). In average the ticks most often contained the genetic material of Rickettsia (68.1±2.94 % of tested samples) and Borrelia, (48.3±1.30 %), then, in descending order, – Anaplasma, (13.5±0.88 %), Ehrlichia (9.5±0.76 %) and tick-borne encephalitis virus (3.0±0.47 %). Despite the low proportion of ticks with the virus in natural foci, 68.5 % of the persons, bit by infected ticks on the territories along Baikalian highway, became ill with tick-borne encephalitis. Conversely, with a wide prevalence of Rickettsia and Borrelia in ticks, the tick-borne rickettsiosis was diagnosed in 3.1 % of the victims and borrelioses – in 28.4 %. No significant differences have been revealed between the infection rate of adult ticks and nymphs, and between males and females, except for Borrelia, which more often have been found in females than in males (51.9 % versus 45.7 %). The tendencies of the pathogens prevalence in time and different plots of territory studied and risks for human infection have been revealed. It was shown than more than a half of human tick-borne encephalitis, tick-borne borrelioses and tick-borne rickettsiosis contracting occurs in the closest suburbs of Irkutsk – up to the 30th km of the highway, where the ticks’ abundance is lower, than in more distant areas, in other words are more to do with human behavioral factor than with the vector’s activity.
About the Authors

Меl’nikova Olga Vitalyevna, Doctor of Sciences (Biology), Senior Research Scientist, Irkutsk Anti-Plague Research Institute of Siberia and Far East of Rospotrebnadzor, 78, Trilisser st., Irkutsk, 664047, Russian Federation, e-mail: melnikovaovit@gmail.com

Adelshin Renat Viktorovich, Candidate of Sciences (Biology), Senior Research Scientist, Irkutsk Anti-Plague Research Institute of Siberia and Far East of Rospotrebnadzor, 78, Trilisser st., Irkutsk, 664047, Russian Federation, e-mail: adelshin@gmail.com

Vershinin Evgeniy Aleksandrovich, Candidate of Sciences (Biology), Research Scientist, Irkutsk Anti-Plague Research Institute of Siberia and Far East of Rospotrebnadzor, 78, Trilisser st., Irkutsk, 664047, Russian Federation, e-mail: eaverschinin@mail.ru

Lopatovskaya Kristina Viktorovna, Junior Research Scientist, Irkutsk Anti-Plague Research Institute of Siberia and Far East of Rospotrebnadzor, 78, Trilisser st., Irkutsk, 664047, Russian Federation, e-mail: krislopatovskaya@mail.ru

Trushina Yulia Nikolaevna, Laboratory Physician, Bokhan District Hospital, 18,1-ya Klinicheskaya st., Bokhan settl., Irkutsk region, 669311, Russian Federation, e-mail: trushinauylia@yandex.ru

Andaev Evgeny Ivanovich, Doctor of Sciences (Medicine), Deputy Director, Head of Laboratory, Irkutsk Anti-Plague Research Institute of Siberia and Far East of Rospotrebnadzor, 78, Trilisser st., Irkutsk, 664047, Russian Federation, e-mail: e.andaev@gmail.com

For citation
Mel’nikova O. V., Adel’shin R. V., Vershinin E. A., Lopatovskaya K. V., Trushina Yu. N., Andaev E. I. Baikalian Highway as a High-Risk Area for Transmissible Tick-Borne Infections. The Bulletin of Irkutsk State University. Series Biology. Ecology, 2023, vol. 46, pp. 29-43. https://doi.org/10.26516/2073-3372.2023.46.29 (in Russian)
Keywords
Ixodid ticks, tick-borne encephalitis virus, Borrelia, Anaplasma, Ehrlichia, Rickettsia, Baikalian highway.
UDC
576.895.42:595.421:578.4
DOI
https://doi.org/10.26516/2073-3372.2023.46.29
References

Aitov K.A. K epidemiologii kleshchevogo entsefalita v Irkutskoi oblasti [To the epidemiology of tick-borne encephalitis in Irkutsk Region]. Zhurnal infektsionnoi patologii, 2018, vol. 23, no. 1-4, pp. 7-8. (in Russian)

Mel’nikova O.V., Trushina Yu.N., Adel’shin R.V., Andaev E.I., Leonova G. I. Analysis of effectivity of the tick-borne encephalitis virus detection methods in Ixodid ticks. Clinical Laboratory Diagnostics, 2021, vol. 66, no. 4, pp. 237-241. (in Russian). http://doi.org/10.51620/0869-2084-2021-66-4-237-241

Andaev E.I. Nauchno-organizatsionnye osnovy epidemiologicheskogo nadzora za prirodnoochagovymi i osobo opasnymi virusnymi infektsiyami v Vostochnoi Sibiri [Scientific and organization basis of epidemiological surveillance for natural-foci and particularly dangerous viral infections in Eastern Siberia: Doctor in Medicine Dissertation Abstract]. Irkutsk, 2009. 324 p. (in Russian)

Botvinkin A.D., Mel'nikova O.V., Danchinova G.A. Raspredelenie infitsirovannykh virusom kleshchevogo entsefalita kleshchei vdol' lineinogo uchetnogo marshruta [Distribution of the ticks infected with tick-borne encephalitis virus, along linear counting route]. Med. Parasitol. Parasit. Dis., 1996, no. 3. pp. 24-28. (in Russian)

Verzhutskii D.B., Verzhutskaya Yu.A. Predvaritel'nye rezul'taty izucheniya prostranstvennoi organizatsii naseleniya taezhnogo kleshcha Ixodes persulcatus po Baikal'skomu traktu (Yuzhnoe Pribaikal'e) [Initial results of the taiga tick Ixodes persulcatus spatial organization along Baikalsky highroad (Southern Cisbaikalia]. Baikal. Zool. J., 2015, no. 1 (16), pp. 71-74. (in Russian)

Chumachenko I.G., Loginovskaja A.A., Bogomazova O.L, Borisov V.A., Aitov K.A., Malov I.V., Danchinova G.A., Kozlova I.V. Izmenenija v strukture zabolevaemosti kleshhevym jencefalitom naselenija Irkutskoj oblasti v 1993-2003 gg. [Changes in the structure of tick-borne encephalitis morbidity in Irkutsk region population]. Bull. ESSC SB RAMS, 2004, no. 1 (3), pp. 170-173. (in Russian)

Yakovchits N.V., Andaev E.I., Bondarenko E.I., Trushina Yu.N., Kotova I.V., Pyrinova G.B., Vetkina T.G., Baranova S.G., Topychkanova N.G., Ofitserov V.I. Otsenka diagnosticheskoi znachimosti kompleksnogo vyyavleniya markerov infektsii, perenosimykh kleshchami, u patsientov, postradavshikh ot ikh prisasyvaniya [Evaluation of diagnostic significance of complex detection of the tick-borne infection markers in the patients suffered from its bites]. Infectious diseases: news, views, education, 2016, no. 4. pp. 55-65. (in Russian)

Mel'nikova O.V., Vershinin E.A., Korzun V.M., Lesnykh S.I., Sidorova E.A., Andaev E I. Primenenie GIS-tehnologij v sravnitel'nom analize zabolevaemosti transmissivnymi kleshhevymi infekcijami (na primere goroda Irkutska) [Using of GIS technology in comparative analysis of tickborne infections morbidity (on the example of Irkutsk city)]. Geography and Natural Resources, 2014, no. 3, pp. 164-172. (in Russian)

Vershinina T.A., Vershinin A.A., Zazulya G.G., Malyshev Yu.S., Lobanova T.A., Revina E.N., Popova A.I. Rekreacionnaya, landshaftno-ehpidemiologicheskaya harakteristika prigorodnoj zony g. Irkutska [Recreational, landscape and epidemiological characteristics of the Irkutsk city suburbs]. Irkutsk Inst. Geogr. SB AS USSR. Irkutsk, 1991, 121 p. Dep. man. VINITI, № 4442-В91 (in Russian)

Mel'nikova O.V., Vershinin E.A., Korzun V.M., Nikitin A.Ya., Verzhutskaya Yu.A. Rol melkih mlekopitajushhih raznyh vidov v prokormlenii preimaginal'nyh stadij tajozhnogo kleshha v Pribajkal'e [Role of different small mammals species in feeding of the taiga tick preimaginal stages in Pribaikalie]. Bull. Irkutsk St. Univ. Ser. Biol. Ecol., 2015, vol. 11, pp. 93-104. (in Russian)

Gorin O.Z., Malykh T.K., Kovsharov A.F., Osodoev Yu.P. Sovremennye osobennosti jepidemiologii kleshhevogo jencefalita v Irkutskoj oblasti. [Modern features of tick-borne encephalitis epidemiology in Irkutsk Region]. Jetiologija, jepidemiologija i diagnostika infekcionnyh zabolevanij Vostochnoj Sibiri [Etiology, epidemiology and diagnostics of infectious diseases in Eastern Siberia]. Irkutsk, Irkutsk Inst. Epidemiol. Microbiol. Publ., 1992. pp. 33-43. (in Russian)

Filippova N.A. Iksodovye kleshchi podsem. Ixodinae [Ixodid ticks of subfamily Ixodinae (Fauna of USSR. Arachnoidea IV (4)]. St.-Petersb., Nauka Publ., 1977, 396 p. (in Russian)

Filippova N.A. Iksodovye kleshchi podsem. Ambliomminae [Ixodid ticks of subfamily Ambliomminae. (Fauna of Russia and neighboring countries. Arachnoidea IV (5)]. St.-Petersb., Nauka Publ., 1997, 436 p. (in Russian)

Shchepin A.Yu., Solntsev I.G. Zarazhennost taezhnykh kleshchei vozbuditelem bolezni Laima na yuge Vostochnoi Sibiri [The Lyme disease infection rate of taiga ticks in the South of Eastern Siberia]. Aktual'nye voprosy infektsionnoi patologii [Actual problems of infectious pathology: Proc. Sci. Conf., Irkutsk, Russia]. Irkutsk, Irkutsk St. Medic. Inst. Publ., 1993, Pt. II, pp. 182-183. (in Russian)

Evolyutsiya kleshchevogo entsefalita (s momenta otkrytiya vozbuditelya po nastoyashchee vremya) [Evolution of tick-borne encephalitis (from the discovery of the pathogen to the present)]. Pogodina V.V., Ishmukhametov A.A. (Eds.). Tver, TPF Publ., 2021, 344 p. (in Russian)

Lagunova E.K., Liapunova N.A., Tuul D., Otgonsuren G., Nomin D., Erdenebat N., Abmed D., Danchinova G.A., Sato K., Kawabata H., Khasnatinov M.A. Co‑infections with multiple pathogens in natural populations of Ixodes persulcatus ticks in Mongolia. Parasites & Vectors, 2022, vol. 15, pp. 236. https://doi.org/10.1186/s13071-022-05356-x

Melnikova O.V., Adelshin R.V., Trushina Y.N., Yakovchits N.V., Andaev E.I. Identification of the Spectrum of Pathogens in Ixodid Ticks from Natural Co-Infection Foci of the Baikal Region. Entomol. Rev., 2019, vol. 99, no. 2, pp. 262-273. https://doi.org/10.1134/S001387381902012X

Melnikova O.V., Vershinin E.A., Verzhutskaya Yu.A., Korzun V.M., Adelshin R.V., Trushina Yu.N., Andaev E.I. Multi-Year Monitoring of the Tick–Pathogen System in Natural Foci of TickBorne Encephalitis in the Suburbs of Irkutsk. Entomol. Rev., 2021, vol. 101, no. 9, pp. 1471-1484.https://doi.org/10.1134/S0013873821090232

Khasnatinov M.A., Danchinova G.A., Takano A. Kawabata H., Ohashi N., Masuzawa T. Prevalence of Borrelia miyamotoi in Ixodes persulcatus in Irkutsk City and its neighboring territories, Russia. Ticks Tick Borne Dis., 2016, vol. 7, no. 2, pp. 394-397. https://doi.org/10.1016/j.ttbdis.2015.12.016

Khasnatinov M.A., Danchinova G.A., Liapunov A.V., Manzarova E.L., Petrova I.V., Liapunovа (Bolotova) N.A., Solovarov I. S. Prevalence of Tick-Borne Pathogens in Hard Ticks That Attacked Human Hosts in Eastern Siberia. Int. J. Biomed., 2017, vol. 7, no. 4, pp. 307-309.https://doi.org/10.21103/Article7(4)_OA7

Rar V.A., Livanova N.N., Panov V.V., Kozlova I.V., Pukhovskaya N.M., Vysochina N.P., Tkachev S.E., Ivanov L.I. Prevalence of Anaplasma and Ehrlichia species in Ixodes persulcatus ticks and small mammals from different regions of the Asian part of Russia. Int. J. Med. Microbiol., 2008, vol. 298, suppl. 1, pp. 222-230. https://doi.org/10.1016/j.ijmm.2008.01.001

Roux V., Fournier P.-E., Raoult D. Differentiation of Spotted Fever Group Rickettsiae by Sequencing and Analysis of Restriction Fragment Length Polymorphism of PCR Amplified DNA of the Gene Encoding the Protein rOmpA. J. Clinic. Microbiol., 1996, vol. 34, no. 9, pp. 2058-2065.https://doi.org/10.1128/jcm.34.9.2058-2065.1996

Roux V., Raoult D. Phylogenetic analysis of members of the genus Rickettsia using the gene encoding the outer-membrane protein rOmpB (ompB). Int. J. Syst. Evol. Microbiol., 2000, vol. 50, pp. 1449-1455.https://doi.org/10.1099/00207713-50-4-1449

Khasnatinov M.A., Liapunov A.V., Manzarova E.L., Petrova I.V., Danchinova G.A., Kulakova N.V. The Diversity And Prevalence of Hard Ticks Attacking Human Hosts in Eastern Siberia (Russian Federation) with First Description of Invasion of Non-Endemic Tick Species. Parasitol. Res., 2016, vol. 115, no. 2, pp. 501-510. https://doi.org/10.1007/s00436-015-4766-7


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