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«IZVESTIYA IRKUTSKOGO GOSUDARSTVENNOGO UNIVERSITETA». SERIYA «BIOLOGIYA. ECOLOGIYA»
«THE BULLETIN OF IRKUTSK STATE UNIVERSITY». SERIES «BIOLOGY. ECOLOGY»
ISSN 2073-3372 (Print)

List of issues > Series «Biology. Ecology». 2023. Vol 44

Effect of Peptides from Honeybee Products on Rats Behavior under Conditions of Chronic Stress

Author(s)
S. V. Klychenkov, A. D. Kruchinina, O. A. Levashova, S. S. Gamzin
Abstract
Chronic stress is one of the causes of depression. Various daily factors, accumulating, can lead to the formation of a constant state of stress and anxiety. In this study, we measured the ability of peptides of royal jelly, drone brood, and bee honey up to 5 kDa to reduce anxiety under chronic stress conditions in male Wistar rats. To do this, the animals of the control and experimental groups were subjected to random stress factors for 14 days (2 factors per day), while simultaneously receiving 0.9% NaCl solution (control group) or aqueous solutions of the corresponding peptides intranasally at a concentration of 300 μg/kg of body weight (peptides were obtained by a combination of ultrafiltration, ion exchange chromatography and gel-filtration). After 14 days, the level of anxiety in animals of all groups was measured using such behavioral tests as “Open field”, “Forced swimming” and “Light-dark box”. It was found that in the OF test, animals treated with royal jelly and drone brood peptides show shorter freezing time, higher horizontal and vertical locomotor activity, spend more time on research activities and grooming; in the FST animals of the same groups spent more time actively swimming; in the LDB test animals of the same groups spent more time in the illuminated part of the experimental setup and crossed the border between the dark and light parts more times. The results indicate that rats treated with royal jelly and drone brood peptides have lower levels of stress and anxiety compared to animals that received 0.9% NaCl solution (control group) or a solution of bee honey peptides. After conducting behavioral tests, the animals were withdrawn from the experiment and the concentration of corticosterone and ACTH was determined in the blood serum. It was found that there is no any changes in hormone concentrations between rats from control or experimental groups. Probably, the peptides of drone brood and royal jelly have an anxiolytic effect, without affecting the work of the hypothalamic-pituitary-adrenal axis, but acting through intracerebral molecular targets.
About the Authors

Klychenkov Sergey Viktorovich, Senior Lecturer, Penza State University, 40, Krasnaya st., Penza, 440026, Russian Federation, e-mail: 79048510599@ya.ru

Kruchinina Anastasia Dmitrievna, Candidate of Sciences (Biology), Associate Professor of the Department, Penza State University, 40, Krasnaya st., Penza, 440026, Russian Federation, e-mail: a.d.kruchinina@mail.ru

Levashova Olga Anatolyevna, Candidate of Sciences (Biology), Assistant Professor, Senior Research Scientist, Penza Institute of Advanced Medical Training – Branch of Russian Medical Academy of Continuing Professional Education, 8A, Stasov st., Penza, 440060, Russian Federation, e-mail: kld@piuv.ru

Gamzin Sergey Sergeevich, Candidate of Science (Biology), Associate Professor, Penza State University, 40, Krasnaya st., Penza, 440026, Russian Federation, e-mail: s.s.gamzin@yandex.ru

For citation
Klychenkov S.V., Kruchinina A.D., Levashova O.A., Gamzin S.S. Effect of Peptides from Honeybee Products on Rats Behavior under Conditions of Chronic Stress. The Bulletin of Irkutsk State University. Series Biology. Ecology, 2023, vol. 44, pp. 68-77. https://doi.org/10.26516/2073-3372.2023.44.68 (in Russian)
Keywords
beekeeping products peptides, open field, forced swimming, royal jelly peptides, drone brood peptides, honey peptides, chronic stress.
UDC
612.8+57.024+577.112
DOI
https://doi.org/10.26516/2073-3372.2023.44.68
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