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«IZVESTIYA IRKUTSKOGO GOSUDARSTVENNOGO UNIVERSITETA». SERIYA «BIOLOGIYA. ECOLOGIYA»
«THE BULLETIN OF IRKUTSK STATE UNIVERSITY». SERIES «BIOLOGY. ECOLOGY»
ISSN 2073-3372 (Print)

List of issues > Series «Biology. Ecology». 2022. Vol 42

Effect of Sodium and Potassium Halides on Growth of Bifidobacterium bifidum Culture

Author(s)
А. S. Pendyukhova, А. А. Pristavka, V. L. Mikhailenko, Y. E. Martovitskaya, G. V. Yurinova, V. P. Salovarova
Abstract
Halide ions are a type of anions that are commonly found in the environment, have important physiological functions, and are used in industry. However, their high content is harmful to the environment and human health, largely depending on the status of the endogenous microbiota. The aim of this work is to research the features of the influence of halides on Bifidobacterium bifidum growth in vitro. The dependence of biomass accumulation in the stationary phase on the content of sodium and potassium halides in thioglycol medium at different concentrations (0,001, 0,01, 0,1 and 1 M) was studied. Empirical values were fitted with dose-response model curves. Two-factor PERMANOVA was shown that the shape of the curves significantly (p<0,01) depends on the type of anion, type of cation, salt concentration and the interaction of factors “type of anion / salt concentration”. Moreover, the biomass of bacteria is often lower in the presence of potassium salts than similar sodium ones. As a result, model curves for different salts has various form (logistics, exponential and hyperbolic). Fluorides and chlorides inhibited biomass growth at all concentrations compared to control. Bromides and iodides at 0,001–0,01 M stimulated the accumulation of biomass to 20–50%. This effect can be used in the food industry, agriculture and healthcare. At a concentration of 0,001 M, a significant regression relation was shown between the atomic mass of the halogen and the fitted curve value of biomass. This dependence probably demonstrates effect atomic radius or/and electronegativity halogens for their penetration into the cell and interaction with cellular targets. The result dependencies do not correspond with state sanitary standards for halide ions. These regulations for fluorides (~10–4 M) and chlorides (10–2 M) roughly match to the survival limit of bifidobacteria. However, more strong limits for bromides (~10–6 M) and iodides (~10–6 M) do not correlate with the biological response of bifidobacteria. In addition, the sanitarian standards halogenids do not take into account the modifying effect of cations which can be important.
About the Authors

Pendyukhova Anna Sergeevna, Postgraduate, Irkutsk State University, 1, K. Marx st., Irkutsk, 664003, Russian Federation, e-mail: annapend@yandex.ru

Pristavka Aleksey Alexandrovich, Candidate of Sciences (Biology), Associate Professor, Irkutsk State University, 1, K. Marx st., Irkutsk, 664003, Russian Federation, e-mail: pristavk@gmail.com

Mikhailenko Valentina Lvovna, Candidate of Sciences (Chemistry), Associate Professor, Irkutsk State University, 1, K. Marx st., Irkutsk, 664003, Russian Federation, e-mail: mival63@gmail.com

Martovitskaya Yulia Evgenievna, Postgraduate, Irkutsk State University, 1, K. Marx st., Irkutsk, 664003, Russian Federation, e-mail: julia.marttt@gmail.com

Yurinova Galina Valerievna, Candidate of Sciences (Biology), Associate Professor, Irkutsk State University, 1, K. Marx st., Irkutsk, 664003, Russian Federation, e-mail: yurinova@yandex.ru

Salovarova Valentina Petrovna, Doctor of Sciences (Biology), Professor, Head of Department, Irkutsk State University, 1, K. Marx st., Irkutsk, 664003, Russian Federation, e-mail: vsalovarova@gmail.com

For citation
Pendyukhova А.S., Pristavka А.А., Mikhailenko V.L., Martovitskaya Y.E., Yurinova G.V., Salovarova V.P. Effect of Sodium and Potassium Halides on Growth of Bifidobacterium bifidum Culture. The Bulletin of Irkutsk State University. Series Biology. Ecology, 2022, vol. 42, pp. 25-36. https://doi.org/10.26516/2073-3372.2022.42.25 (in Russian)
Keywords
halide ions, Bifidobacterium bifidum, dose-response relationship, modeling.
UDC
579.26+57.087.1
DOI
https://doi.org/10.26516/2073-3372.2022.42.25
References

Adambekov D.A., Khamzaev B.D., Adambekova A.D. Mikrobiota cheloveka i ee znachenie [Human microbiota and its significance (literature review)]. Vestnik of KSMA named after I.K. Akhunbaev, 2019, no. 5-6, pp. 44-45. (in Russian)

Vasil'ev A.L., Tarasov A.S., Guseva L.D. Sovremennye metody obezzarazhivaniya vody [Modern methods of water disinfection]. Privolzhsky Sci. J., 2022, no. 3, pp. 83-89. (in Russian)

Chashina E.R., Efremenko Z.A., Salovarova V.P., Gavrikov D.E., Pristavka A.A. Gidroliz tsellyulozy fermentnym kompleksom Trichoderma viride v prisutstvii ftorida natriya: vliyanie struktury substrata i sorbtsionnoi aktivnosti tsellyulaz [Hydrolysis of cellulose by Trichoderma viride enzyme complex in the presence of sodium fluoride: influence of substrate structure and sorption activity of cellulases]. News of Universities. Applied Chemistry and Biotechnology, 2020, vol. 10, no. 2 (33), pp. 261-273. (in Russian)

Gornostaeva E.A., Fuks S.L. Vliyanie ftorsoderzhashchikh soedinenii na zhivye organizmy (obzor) [Influence of fluorinated compounds on living organisms (review)]. Theoretical and Applied Ecology, 2017, no. 1, pp. 14-24. https://doi.org/10.25750/1995-4301-2017-1-014-024

Gulmakhmadzoda Z.G. Standartizatsiya mazi brom tiadizol pirimidinovoi 1% [Standardization of Bromine Thiadisole Pyrimidine Ointment 1%]. Rep. NAS of Tajikistan, 2021, vol. 64, no. 11-12, pp. 712-721. (in Russian)

Evdokimova G.A., Mozgova N.P. Otsenka zagryazneniya pochv i rastenii v zone vozdeistviya gazovozdushnykh vybrosov alyuminievogo zavoda [Assessment of soil and plant pollution in the area affected by air-gas emissions from an aluminum smelter]. Theoretical and Applied Ecology, 2015, no. 4, P.64-68. (in Russian)

Zhukova A.G., Mikhailova N.N., Kazitskaya A.S. Sovremennye predstavleniya o molekulyarnykh mekhanizmakh fiziologicheskogo i toksicheskogo deistviya soedinenii ftora na organizm [Contemporary concepts of molecular mechanisms of the physiological and toxic effects of fluorine compounds on an organism]. Medicine in Kuzbass, 2017, vol. 16, no. 3, pp. 2-7. (in Russian)

Popov I.V., Safronenko A.V., Mazanko M.S., Tyaglivyi A.S., Golovin S.N., Popov I.V., Ermakov A.M. Istoriya primeneniya iodsoderzhashchikh veshchestv v aseptike i antiseptike [History of the use of iodine-containing substances in asepsis and antiseptics]. Veter. Pathol., 2021, vol. 4, no.78, pp. 76-83. (in Russian)

Klimenko I.V., Zhuravleva T.S. Bromirovanie grafitirovannykh pekovykh volokon [Bromination of graphitized pitch fibers]. Readings Name of A.I. Bulatov, 2020, vol. 5, pp. 110-113. (in Russian)

Kuzin A.A., Kuzina D.A, Grunskaya V.A. Vliyanie ioda i zheleza na zakvasochnye kul'tury [Influence of iodine and iron on starter cultures]. Dairy Industry, 2014, no. 9, pp. 38-40.

Kashparova V.P., Shubina E.N., Il'chibaeva I.B., Kashparov I.I., Zhukova I.Yu., Kagan E.Sh. Prevrashchenie spirtov v nitrily v usloviyakh elektrokataliticheskogo okisleniya [Transformation of alcohols into nitriles under conditions of electrocatalytic oxidation]. Electrochemistry, 2020, vol. 56, no. 5, pp. 446-449. https://doi.org/10.31857/S0424857020050059

Safina D.D., Abdulkhakov S.R., Amirov N.B. Mikrobiota kishechnika i ee znachenie dlya zdorov'ya cheloveka [Gut microbiota and its importance for human health]. Bul. Contemp. Clinic. Med., 2021, vol. 14, no. 5, pp. 81-94. (in Russian)

Chereshnev V.A., Poznyakovskii V.M. Faktor pitaniya i evolyutsionno-geneticheskoe formirovanie kishechnoi mikroflory: znachenie dlya sokhraneniya immuniteta i zdorov'ya [Nutrition factor and evolutionary-genetic formation of intestinal microflora: importance for the preservation of immunity and health]. Food Industry, 2020, vol. 5, no. 3, pp. 5-16. (in Russian)

Wang W., Li R., Tan J., Luo K., Yang L., Li H., Li Y. Adsorption and leaching of fluoride in soils of China. Fluoride, 2002, vol. 35, no. 2, pp. 122-129.

Aiuppa A., Baker D.R., Webster J.D. Halogens in volcanic systems. Chem. Geol., 2009, no. 263, pp. 1-18. https://doi.org/10.5281/zenodo.3378010

Anderson M.J. Permutational Multivariate Analysis of Variance (PERMANOVA). Statistics Reference Online, 2017, no. 14, pp. 1-15. https://doi.org/10.1002/9781118445112.stat07841

Drossman D.A. Functional Gastrointestinal Disorders: History, Pathophysiology, Clinical Features and Rome IV. Gastroenterology, 2016, vol. 150, no. 6, pp. 1262-1279. https://doi.org/10.1053/j.gastro.2016.02.032

Al-Adilah H., Feiters M. C., Carpenter L. J., Kumari P., Carrano C. J., Al-Bader D., Küpper F. C. Halogens in Seaweeds: Biological and Environmental Significance. Phycology, 2022, vol. 2. no 1. pp. 132-171. https://doi.org/10.3390/phycology2010009

Hammer O., Harper D.A., Ryan P.D. PAST: Paleontological Statistics Software Package for Education and Data Analysis. Palaeontologia Electronica, 2001, vol. 4, no. 1. pp. 9.

Jayaraj R., Megha P., Sreedev P. Organochlorine pesticides, their toxic effects on living organisms and their fate in the environment. Interdiscip. Toxicol., 2016, vol. 9, no. 3-4. pp. 90-100.

Myers J.A., Curtis B.S., Curtis W.R. Improving accuracy of cell and chromophore concentration measurements using optical density. BMC Biophys., 2013, vol. 6, no. 4, https://doi.org/10.1186/2046-1682-6-4

Ojima I. Fluorine in medicinal chemistry and chemical biology. Oxford: Blackwell Publishing, 2009, 624 p. https://doi.org/10.1002/9781444312096

Gagliardi A., Totino V., Cacciotti F., Iebba V., Neroni B., Bonfiglio G., Trancassini M., Passariello C., Pantanella F., Schippa S. Rebuilding the Gut Microbiota Ecosystem. Int. J. Environ. Res. Public Health., 2018, vol. 15, no, 8. pp. 1679. https://doi.org/10.3390/ijerph15081679

Wu D., Hu Y., Liu Y., Zhang R. Review of chloride ion detection technology in water. Appl. Sci., 2021, vol. 11, no. 23, 11137. https://doi.org/10.3390/app112311137

Fang W.Y., Ravindar L., Rakesh K.P., Manukumar H.M., Shantharam C.S., Alharbi N.S., Qin H.L. Synthetic approaches and pharmaceutical applications of chloro-containing molecules for drug discovery: A critical review. J. Med. Chem., 2019, vol. 1, no. 173. pp. 117-153. https://doi.org/10.1016/j.ejmech.2019.03.063

Wang J., Sui M., Yuan B. Inactivation of two Mycobacteria by free chlorine: Effectiveness, influencing factors, and mechanisms. Science of the Total Environment, 2019, no. 648, pp. 271-184. https://doi.org/10.1016/j.scitotenv.2018.07.451


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