Human papillomavirus (HPV) appears to be significant danger for mankind because of having very high widespread and might revealing the pandemy of papillomatoses. To the present time, about 200 types of HPV have been found, 40 types from which announced as high-risk cancerogenic. It was learned that papillomaviruses are very resistant to impacts of environmetal factors because they posseses many possibilities for long surviving in polluted water conditions. Nevertheless, the infection of HPV through the contact with contaminated water areas still does not to be prove with strong scientific data, foreign scientists are seriously bothering about facts of finding of DNA of human papillomaviruses in sewage water and in different deteriorated water objects. Our opinion is based on the necessity of the comprehensive investigation of the presence of the high-risk oncogenic types of HPV in sewage water. The present study is devoted for the first time to the data of finding of DNA of several high-risk oncogenic HPV in sewage water. Three pairs of primers were used in the study, between them 2 pairs of universal primers (GP5+/6+) which were very specific to nucleic sequences encoded the main structural element of the HPV capsid, the “late” protein L1 (HPV6 L1, HPV11 L1 and HPV16 L1). As the result, the presence of DNA of high-risk oncogenic papillomaviruses of types 6 and 16 was shown in sewage water. Further, the scope of investigations directed to finding of the presence of HPV components is planned to be arranged not only for sewage water but for other water areas of Irkutsk Region of East Siberia as well around water areas of the Lake Baikal.

Stolbikov Aleksey Sergeevich, Candidate of Sience (Biology), Research Scientist, Siberian Institute of Plant Physiology and Biochemistry SB RAS, 132, Lermontov st., Irkutsk, 664033, Russian Federation; Assistant Professor, Irkutsk State University, 1, K. Marx st., Irkutsk, 664033, Russian Federation, e-mail: valkir5@yandex.ru 

Salyaev Ryurik Konstantinovich, Corresponding Member for RAS, Doctor of Science (Biology), Advisor for RAS, Siberian Institute of Plant Physiology and Biochemistry SB RAS, 132, Lermontov st., Irkutsk, 664033, Russian Federation, e-mail: salyaev@sifibr.irk.ru 

Rekoslavskaya Nataliya Igorevna, Doctor of Sciences (Biology), Principal Research Scientist, Siberian Institute of Plant Physiology and Biochemistry SB RAS, 132, Lermontov st., Irkutsk, 664033, Russian Federation, e-mail: rekoslavskaya@sifibr.irk.ru

Stolbikov A.S., Salyaev R.K., Rekoslavskaya N.I. Study of the Presence of Human Papillomavirus (HPV) DNA in Domestic Sewages. The Bulletin of Irkutsk State University. Series Biology. Ecology, 2020, vol. 31, pp. 93-98. https://doi.org/10.26516/2073-3372.2020.31.93 (in Russian)

Di Bonito P., Libera S.D., Petricca S., Iaconelli M., Sanguinetti M., Graffeo R., Accardi L., La Rosa G. A large spectrum of alpha and beta papillomaviruses are detected in human stool samples. J. Gen. Virol., 2015, vol. 96, pp. 607-613. https://doi.org/10.1099/vir.0.071787-0

Fratini M., Di Bonito P., La Rosa G. Oncogenic Papillomavirus and Polyomavirus in Water Environments: Is There a Potential for Waterborne Transmission? Food Environ. Virol., 2014, vol. 6, pp. 1-12. https://doi.org/10.1007/s12560-013-9134-0

Human papillomavirus (HPV) and cervical cancer. World Health Organization review. 24.01.2019. https://www.who.int/news-room/fact-sheets/detail/human-papillomavirus-(hpv)-and-cervical-cancer

La Rosa G., Fratini M., Accardi L., D’Oro G., Libera S.D., Muscillo M., Di Bonito P. Mucosal and Cutaneous Human Papillomaviruses Detected in Raw Sewages. PLOS ONE, 2013, vol. 5, no. 1, рр. e52391. https://doi.org/10.1371/journal.pone.0052391

Cantalupo P.G., Calgua B., Zhao G., Hundesa A., Wier A.D., Katz J.P., Grabe M., Hendrix R.W., Girones R., Wang D., Pipas J.M. Raw Sewage Harbors Diverse Viral Populations. mBio, 2011, vol. 2, no. 5, pp. e00180-11. https://doi.org/10.1128/mBio.00180-11

Symonds E.M., Griffin D.W., Breitbart M. Eukaryotic Viruses in Wastewater Samples from the United States. Appl. Environ. Microbiol., 2009, vol. 75, no. 5, pp. 1402-1409. https://doi.org/10.1128/AEM.01899-08

de Roda Husman A.M., Walboomers J.M., van den Brule A.J., Meijer C.J., Snijders P.J. The use of general primers GP5 and GP6 elongated at their 3 ends with adjacent highly conserved sequences improves HPV detection. J. Gen. Virol., 1995, vol. 76, no. 4, pp. 1057-1062. https://doi.org/10.1099/0022-1317-76-4-1057