The thin-layer chromatography method is convenient and economical in the performance, is allowed to conduct a rapid analysis of the composition of complex lipid mixtures and apply it to assess of the quality of the seeds, favorable conditions for their growth, etc. At this stage of the study, a solvent system was selected for a more complete separation of neutral lipids and phospholipids of the pine seeds Pinus silvestris Ledeb, which is one of the dominant forest-forming species in Irkutsk region and the most important raw material. TLC carried out a preliminary assessment of the component composition of lipid fractions. The solvent system hexane-diethyl ether-acetic acid (70: 30:1) was carried out by the optimal system for separation of neutral lipids of pine seeds. The best results were obtaned separating of phospholipids and using the chloroform-methanol-ammonia system (65:35:3). Ten classes of NL and nine fractions of PL were revealed.

1. Ipatova O.M., Prozorovskaya N.N., Torkhovskaya T.N., Baranova B.C., Guseva D.A. Biologicheskaja aktivnost soevyh fosfolipidov [Bioactivity of soybean phospholipids]. Biomeditsinskaya khimiya [Biomedical Chemistry], 2004, vol. 50, no. 5, pp. 436-450. (in Russian).

2. Bobrov E.G. Lesoobrazujushhie hvojnye SSSR [Forest Forming Coniferous of the USSR]. Moscow, Nauka Publ., 1978, 188 p. (in Russian).

3. Boldyrev A.A., Kjajvjarjajnen E.I., Iljuha V.A. Biomembranologija [Biomembranology]. Petrozavodsk, Karelian NC RAN Publ., 2006, 226 p. (in Russian).

4. Vojtjuk V.P., Koritan Z.N. Ispytatel'nye i sortoispytatel'nye kul'tury sosny obyknovennoj na Volyni [Strain-testing of Scots Pine in Volyn’]. Kiev, 1999, 70 p.

5. Safonova E.F., Nazarova A.A., Selemenev V.F. Vybor optimal'nyh parametrov razdelenija fosfolipidov v tonkom sloe sorbenta [Optimal Parameters of Separation of Phospholipids in Thin Sorbent Layer]. Himiko-farmacevticheskij zhurnal [Pharmaceutical Chemistry Journal], 2002, vol. 36, no. 4, pp.41-43. (in Russian).

6. Golovkin B.N. Biologicheski aktivnye veshhestva rastitel'nogo proishozhdenija [Plant-Based Bioactive Substances]. Moscow, Nauka Publ., 2001, 764 p. (in Russian).

7. Dejneka V.I., Dejneka L.A. Issledovanie trigliceridnogo sostava masla Pinus sibirica Du Tour [Triglycerides in Pinus sibirica Oil]. Himija prirodnyh soedinenij [Chemistry of Natural Compounds], 2003, no. 2, pp. 126-128.

8. Donskaja L.I., Muzalevskaja O.V. Sostav nejtral'nyh lipidov i fosfolipidov semjan Pinus sibirica Du Tour [Neutral Lipids and Phospholipids in Pinus sibirica Seeds]. Rastitel'nye resursy [Plant Resources], 1994, vol. 3, pp. 64-66. (in Russian).

9. Efremov A.A. Perspektivy malotonnazhnoj pererabotki kedrovyh orehov v produkty pishhevogo i tehnicheskogo naznachenija [Perspectives of Processing of Pinus sibirica Seeds into Food and Technical Raw Products]. Khimija rastitel'nogo syr'ja [Chemistry of plant raw material], 1998, no. 3, pp. 83-86. (in Russian).

10. Makarenko S.P., Konenkina T.A., Putilina T.E., Donskaja L.I., Muzalevskaja O.V. Zhirnokislotnyj sostav lipidov jendosperma i zarodysha semjan Pinus sibirica i P. sylvestris [Lipid Composition of Endosperms and Germs of Pinus sibirica and P. sylvestris Seeds]. Fiziologija rastenij [Plant Physiology], 2008, vol. 55, no. 4, pp. 535-540. (in Russian).

11. Shanskaja A.I., Puchkova S.M., Nedachina N.A., Hmylova G.A. Issledovanie frakcionnogo sostava fosfolipidnyh komponentov liposomalnogo preparata lipoferol s usovershenstvovaniem metoda tonkoslojnoj hromatografii [Fractional Composition of Phospholipid Components in Liposomal Preparation Lipoferol with improved TLH Method]. Gematologija [Gematology], 2014, vol. 15, pp. 718-730. (in Russian).

12. Kolesnikova T.G., Subbotina M.A., Shubenkina N.S. Issledovanie himicheskogo sostava belkovo-lipidnogo produkta iz kedrovyh orehov [Chemical Composition ontent of Protein and Lipid Product Extracted from Siberian Pine Seeds]. Tehnika i tehnologija pishhevyh proizvodstv. [Food Processing: Techniques and Technology], 2013, no. 2, pp. 22-26. (in Russian).

13. Kabanov N.E. Hvojnye derev'ja i kustarniki Dal'nego Vostoka [Coniferous Trees and Shrubs of Far East]. Moscow, Nauka Publ., 1977, 175 p. (in Russian).

14. Kejts M. Tehnika lipidologii: Vydelenie, analiz i identifikacija lipidov [Lipidology: Extraction, Analysis and Identification of Lipids]. Moscow, Mir Publ., 1975, 322 p. (in Russian).

15. Kizeev A.N. Pozicionno-tipovoj i pozicionno-vidovoj sostav triacilglicerinov iz masla semjan podsolnechnika [Triacylglycerol Composition ontent in Oilseed Seed Oil]. Molodoj uchenyj [Young Scientist], 2013, no. 6, pp. 241-244. (in Russian).

16. Kirhner E. Tonkoslojnaja hromatografija [Thin-layer Chromatography]. Moscow, Mir Publ., 1979, 606 p. (in Russian).

17. Kramer D., Kozlovskij T. Fiziologija drevesnyh rastenij [Wood Plants Physiology]. Moscow, Lesnaja promyshlennost' Publ., 1983, 464 p. (in Russian).

18. Alaudinova E.V., Povaljaeva V.A., Mironov P.V. Lipidy meristem lesoobrazujushhih hvojnyh porod Central'noj Sibiri v uslovijah nizkotemperaturnoj adaptacii. 3. Osobennosti obmena nejtral'nyh lipidov meristem pochek Larix sibirica Ledeb., Picea obovata L. i Pinus silvestris L. Khimija rastitel'nogo syr'ja [Chemistry of plant raw material], 2010, no. 1, pp. 67-74. (in Russian).

19. Petrov K.A., Perk A.A., Chepalov V.A., Ohlopkova Zh.M. Osobennosti zhirnokislotnogo sostava nekotoryh rastenij Jakutii v period formirovanija kriorezistentnosti [Characteristics of Fatty Acid Composition of Some Plants of Yakutia during the Development of Cryoresistance]. Vestnik Severo-Vostochnogo federal'nogo universiteta im. M. K. Ammosova [Herald of North-Eastern Federal University], 2011, vol. 8, no. 2, pp. 26-29. (in Russian).

20. Pakhomova I.G., Uspenskii Yu.P. Essentsial'nye fosfolipidy: svoistva i osobennosti [Eccential Phospholipids: Characteristics and Features]. Gastroenterologiya [Gastroenterology], 2010, no. 1, pp. 75-79. (in Russian).

21. Permjakov B. G. Kedr nash sibirskij [Siberian Pine]. Irkutsk, Vostochno-Sibirskoye knizhnoye izdatel’stvo Publ., 1986, 208 p. (in Russian).

22. Sikorskaya A.S., Nazarova A.A., Selemenev V.F. Podbor optimal'nyh uslovij razdelenija fosfolipidnyh kompleksov, poluchennyh iz semjan podsolnechnika [Selection of Optimal Regimes for the Separation Phospholipid Complexes Extracted from Oilseed Seeds]. Sorbcionnye i hromatograficheskie processy [Sorbtion and Chromatographic Processes]. 2009, vol. 9, no. 2, pp. 215-220. (in Russian).

23. Sazonova T.A., Bolondinskii V.K., Pridacha V.B. Ekologo-fiziologicheskaja harakteristika sosny obyknovennoj [Ecological and Physiological Characteristics of Scots Pine]. Petrozavodsk, Verso Publ., 2011, 207 p. (in Russian).

24. Bakhtin Yu.V., Budaeva V.V., Vereshchagin A.L., Egorova E.Yu., Zhukova E.E., Saratikov A.S. Jeffektivnost' ispol'zovanija kedrovogo masla v kompleksnom lechenii bol'nyh s arterial'noj gipertoniej [Effectiveness of Siberian Pine Oil in Complex Therapy of Arterial Hypertension]. Voprosy pitanija [Problems of Nutrition], 2006, vol. 75, no. 1, pp. 51-53. (in Russian).

25. Skakovskii E.D., Tychinskaya L.Yu., Gaidukevich O.A., Kulakova A.N., Petlitskaya N.M. JaMR-analiz masel kedrovogo oreha (Pinus sibirica) i semjan sosny obyknovennoj (Pinus sylvestris L.) [TLS-analysis of Oils in Pinus sibirica and Pinus sylvestris Seeds]. Zhurnal prikladnoi spektroskopii [Journal of Applied Spectroscopy], 2007, vol. 74. pp. 528-532. (in Russian).

26. Wood J.D., Enser M., Fisher A.V., Nute G.R., Sheard P.R., Richardson R.I., Hughes S.I., Whittington F.M. Fatty acid composition of Pinaceae as taxonomic markers. Lipids, 2001, vol. 36, no. 5, pp. 439-451.

27. Folch J., Lees M., Stanley G.H. A simple method for isolation and purification of total lipids from animal tissues. J. Biol. Chem., 1957, vol. 226, no. 1, pp. 497-509.

28. Wolff R.L., Pedrono F., Pasquier E., Marpeau A.M. General characteristics of Pinus spp. seed fatty acid compositions, and importance of delta5-olefinic acids in the taxonomy and phylogeny of the genus. Lipids, 2000, vol. 35, no. 1, pp. 1-22.