List of issues > Series «Biology. Ecology». 2009. Vol. 2
Use of molecular-genetic methods for typification of biridobacteria and the analysis of specific architectonic of intestial microbiocenosis in population of the industrial city
Author(s)
Yu. P. Dzhioev, Е. G. Lamskov, S.М. Popkova, А. G. Leontyeva, Е. B. Rakova, L. V. Mironova, G. V. Jurinova, V. P. Salovarova, A.V. Lyapunov, А. А. Pristavka, P. А. Medvedeva, A. I. Pilueva, A. S. Volokitina
Abstract
For the first time in regional clinical practice the method of the polymerization chain reaction (PCP) for diagnostics of genera and species of bifidobacteria is used. The offered approach is adapted and optimised on conditions of diagnostic standards for working out of a method of identification and typification of bifidobacteria species in a multiplex variant. The received results of specific typification of bifidobacteria in population of an industrial city (Angarsk), somewhat reflect complexity of mechanisms of influence of external and internal factors on formation processes of intestines microbiocenosis, shown in a structural variety of specific architectonic of bifidobacteria in an human organism.
Keywords
Polymerization chain reaction, bifidobacteria, generic and specific probes, identification and typification, disbacteriosis
UDC
616-008.87-07.097.931