List of issues > Series «Biology. Ecology». 2010. Vol. 3
Transformation of tobacco mitochondria by genetic construct with integrative properties and selection of cell lines with transgenic mitochondria based on resistance to antimycin A
Author(s)
A. I. Katyshev, V. N. Shmakov, V. V. Chernikova, Yu. V. Sidorchuk, E. V. Deineko, Yu. M. Konstantinov
Abstract
The delivery by biolistic method of mitochondrial genetic vector conferring resistance to antimycin A to get tobacco transformed mitochondria in vivo was investigated. Callus lines which are resistant to respiratory complex III inhibitor antimycin A were obtained. These cell lines demonstrate high growth rate in the presence of this respiratory inhibitor in comparison with non-transformed lines. We suggest that growth characteristics of selected callus lines are explained by transgene expression in mitochondria of these lines
Keywords
Nicotiana tabacum, callus lines, mitochondrial genetic vector with integrative properties, modified cob gene, biolistic transformation, resistance to antimycin A
UDC
УДК 576.315