Three factors – heat (39 °C, 2 h), mitochondrial inhibitor iodoacetic acid sodium salt (IAS) and infection with the causative agent of a disease ring rot – Clavibacter michiganensis ssp sepedonicus (Cms) (as separately, and in combinations) on accumulation of thermal shockproteins: HSP101, HSP60, HSP17.6 in tissues of potatoes of two varieties Lugovskoy and Lukyanovsky in vitro was examined. It is shown the heat influence (39 °C, 2 h) in increases the content of these at potato plants of both varieties. The greatest increase is noted for HSP101. Infection of plants with a pathogen of Cms is led to the strengthened accumulation of HSP101, HSP60, HSP17.6 in tissues by Lukyanovsky, at Lugovskoy potato the synthesis of the studied proteins wasn't noted. In all options with addition of IAS the increased maintenance of HSP, the brightest effect – at plants of Lukyanovsky was noted. Joint impact of IAS and the increased temperature led to stimulation of accumulation of HSP101 and HSP60 in tissues of Lukyanovsky. At infection of plants and IAS processing jointly the strengthening of HSP accumulation wasn't noted. Influence of all three stressful factors: bacteria, monoiodacetate and the increased temperature in common raised the maintenance of all HSP in potato tissues of both varieties, but it isn't as significant as influence of IAS and temperature with no infection of Cms. In the article is shown for the first time that the processing of IAS activates the accumulation of HSP, it indicates the participation of mitochondria in regulation of HSP expression.