The influence of humate and surfactants on the processes of sorption and desorption of microorganisms from hydrophobizated surfaces is investigated. The experiments showed that the number of cells of microorganisms and spores, that remain associated with the surface of hydrophobic glass slides in the presence in solution of certain concentration Powhumus concentrated humic acid substance or Twin Series of surfactants is lower, than in their absence. In the cases of adding humate or Tween 21 at concentrations of 0.5 and 1 g/l sorption of Y. lipolytica cells and spores of B. thuringiensis was less intense than in their absence. It is shown that Powhumus, Tween 21 and Tween 85 decrease the adhesion of Y. lipolytica cells and spores of B. thuringiensis and increase the desorption of microorganism’s cells and spores from the surfaces of glasses covered by paraffine or silicone gel in concentrations 1 and 2 g/l. At the same concentrations of Powhumus and Tween 21 disturbed films of paraffine on surfaces of glasses. It should be noted that the solution of Twin 85 at a concentration of 1 and 2 g/l did not reduce the number of cells of Y. lipolytica, adsorbed on parafinirovannaja the surface, but on glass, treated silicone gel, in both cases, saw a decrease in their numbers. In this case the surfactant were more active than humic substances.