Until recently it was assumed that the transcriptional activity of genes plays a key role in the regulation of gene expression. Whereas the regulation on the translation level was considered as only an additional step of different proteins balance correction used by cells in changing extracellular and intracellular environment. A large amount of evidence of a crucial contribution of the posttranscriptional regulation, especially in eukaryotes, has collected to the present moment. In this work we identify factors which may influence the expression of genes under the control of the CUP1 promoter at the translational level in yeast Saccharomyces cerevisiae. For this purpose we applied a number of reporter systems allowing quantifying an influence of various factors on the efficiency of translation from  mRNAs bearing 5'-UTR CUP1. This approach allowed us to evaluate the production of proteins in the native isoform in terms of a single cell. The results obtained are important for understanding of the phenomenon of the posttranscriptional regulation of gene expression in eukaryotes and can be applied for the improvement inrecombinant proteins production in biotechnology.