The present paper describes an attempt of bioballistic transformations of tomato calluses to the end of assessment of the potentialities of this method application for the purpose of obtaining plants having some larger content of the target protein. The work initiated represents the first stage, which presumes the application of GUS gene in the assessment of the manipulations applied to the end of obtaining the plants transformed with the use of the target gene with plastid addressing. More than 2 000 calluses have been received and 6 series of bioballistic experiments are made. The transformed calluses of tomato plants were investigated by means of fluorescent and histochemical methods which have shown high percent of successful genetic transformation. The received results demonstrate that bioballistic transformation of calluses can be effectively used for further work with the target gene having plastid addressing.