The intensity of spontaneous luminescence microorganism’s suspension of the pure Baikal culture (Bacillus subtilis, Micrococcus luteus, Nocardia sp., Pseudomonas aeruginosa, Sinedra acus radians), unicellular algae (Scenedesmus quadricauda and Dunaliella salina) and recombinant strain with included lux-operon (Escherichia coli) was compared by means of self-made setup (laboratory photometer, designed in Physical and Technical Institute of Irkutsk State Technical University). The strain E. coli is widely using in bio testing. Lake Baikal microorganism’s luminescence intensity upon recalculation on one cell was 108–104 times lower than that of recombinant E. coli (Imax). Algae’s prolonged luminescence after external lighting was found. It is only 103 times lower than Imax and can give some impact into Baikal water luminescence in the photic zone.